RRC ID 77192
Author Yasutake K, Uemori A, Binh ND, Mizuki E, Ohba M.
Title Identification of parasporin genes in Vietnamese isolates of Bacillus thuringiensis.
Journal Z Naturforsch C J Biosci
Abstract Four genes encoding parasporins, cytotoxins preferentially killing human cancer cells in vitro, were isolated from four Vietnamese strains of Bacillus thuringiensis. Nucleotide sequence analysis revealed that: (1) three genes fall into the two known classes, ps1Aa and ps1Ab, and (2) another one belongs to ps1Ac, a novel gene class established in this study. Upon proteolytic activation, parasporal protein of the organism with ps1Ac exhibited strong cytocidal activity against human cancer cells, HeLa and Hep G2, but not to non-cancer normal cells, UtSMC and HC.
Volume 63(1-2)
Pages 139-43
Published 2008-1-1
DOI 10.1515/znc-2008-1-225
PMID 18386503
MeSH Bacillus thuringiensis / genetics* Bacillus thuringiensis / isolation & purification Bacillus thuringiensis / pathogenicity Endopeptidase K / metabolism Endotoxins / genetics* Humans Molecular Sequence Data Plasmids Polymerase Chain Reaction Vietnam
IF 1.238
Human and Animal Cells HeLa(RCB0007) Hep G2