| RRC ID |
77206
|
| 著者 |
Iyyappan J, Jayamuthunagai J, Bharathiraja B, Saravanaraj A, Praveen Kumar R, Balraj S.
|
| タイトル |
Production of biodiesel from Caulerpa racemosa oil using recombinant Pichia pastoris whole cell biocatalyst with double displayed over expression of Candida antartica lipase.
|
| ジャーナル |
Bioresour Technol
|
| Abstract |
In this study, Caulerpa racemosa oil was used to produce biodiesel by recombinant Pichia pastoris displaying bound (rPp-BL) and secretory lipase (rPp-SL). Collected algae was pre-treated using ultrasonication, microwave and solvent extraction. Defatted C. racemosa was subjected to dilute acid treatment to obtain algal biomass hydrolysate. Both rPp-BL and rPp-SL were cultivated in algal biomass hydrolysate and glycerol. Surfactant treatment was performed on rPp-BL. Screening and optimization of variables were performed for biodiesel production using Plackett Burman design and central composite design, respectively. About 10.64 % (w/w) of algal oil was extracted from C. racemosa. Both rPp-BL and rPp-SL effectively utilized C. racemosa biomass hydrolysate and glycerol. rPp-SL combined with triton X (1.0 % w/v) treated rPp-BL for 3 min improved lipase activity. Methanol to oil ratio, combined whole cell biocatalyst and temperature were significant factors. Under optimum conditions, biodiesel yield reached about 93.64 % after 30 h using developed whole cell biocatalyst.
|
| 巻・号 |
363
|
| ページ |
127893
|
| 公開日 |
2022-11-1
|
| DOI |
10.1016/j.biortech.2022.127893
|
| PII |
S0960-8524(22)01223-8
|
| PMID |
36067897
|
| MeSH |
Biofuels*
Candida / metabolism
Caulerpa* / metabolism
Glycerol / metabolism
Lipase / metabolism
Methanol / metabolism
Methanol / pharmacology
Pichia / genetics
Pichia / metabolism
Saccharomycetales
Solvents / metabolism
Surface-Active Agents / metabolism
|
| IF |
7.539
|
| リソース情報 |
| 一般微生物 |
JCM 10318 |
