RRC ID 77213
Author Kowalik K, Polak-Berecka M, Prendecka-Wróbel M, Pigoń-Zając D, Niedźwiedź I, Szwajgier D, Baranowska-Wójcik E, Waśko A.
Title Biological Activity of an Epilobium angustifolium L. (Fireweed) Infusion after In Vitro Digestion.
Journal Molecules
Abstract The biological activity of an in vitro digested infusion of Epilobium angustifolium (fireweed) was examined in a model system of intestinal epithelial and colon cancer tissues. The content of selected phenolic compounds in the digested aqueous extract of fireweed was determined using HPLC-ESI-QTOF-MS/MS. Biological activity was examined using the human colon adenocarcinoma cell lines HT-29 and CaCo-2 and the human colon epithelial cell line CCD 841 CoTr. Cytotoxicity was assessed by an MTT assay, a Neutral Red uptake assay, May-Grünwald-Giemsa staining, and a label-free Electric Cell-Substrate Impedance Sensing cytotoxicity assay. The effect of the infusion on the growth of selected intestinal bacteria was also examined. The extract inhibited the growth of intestinal cancer cells HT-29. This effect can be attributed to the activity of quercetin and kaempferol, which were the most abundant phenolic compounds found in the extract after in vitro digestion. The cytotoxicity of the fireweed infusion was dose-dependent. The highest decrease in proliferation (by almost 80%) compared to the control was observed in HT-29 line treated with the extract at a concentration of 250 μg/mL. The fireweed infusion did not affect the growth of beneficial intestinal bacteria, but it did significantly inhibit E. coli. The cytotoxic effect of the fireweed extract indicates that it does not lose its biological activity after in vitro digestion. It can be concluded that the fireweed infusion has the potential to be used as a supporting agent in colon cancer therapy.
Volume 27(3)
Published 2022-2-2
DOI 10.3390/molecules27031006
PII molecules27031006
PMID 35164271
PMC PMC8839289
MeSH Antineoplastic Agents, Phytogenic / chemistry* Antineoplastic Agents, Phytogenic / pharmacology Caco-2 Cells Cell Proliferation / drug effects Epilobium / chemistry* HT29 Cells Humans Neoplasms / drug therapy Plant Extracts / chemistry* Plant Extracts / pharmacology Polyphenols / chemistry* Polyphenols / pharmacology
IF 3.267
General Microbes JCM 1513