RRC ID 77222
Author Dewake N, Ma X, Sato K, Nakatsu S, Yoshimura K, Eshita Y, Fujinaka H, Yano Y, Yoshinari N, Yoshida A.
Title β-Glycyrrhetinic acid inhibits the bacterial growth and biofilm formation by supragingival plaque commensals.
Journal Microbiol Immunol
Abstract β-Glycyrrhetinic acid (BGA) is a natural antibacterial agent. Previous studies reported that BGA has antibacterial effects against several bacteria. This study evaluated the effects of BGA on the regulation of supragingival plaque bacteria. First, the minimum inhibitory concentrations (MICs) of BGA against oral bacteria were measured. Next, the minimum concentrations for inhibition of biofilm formation were evaluated against Streptococcus mutans and Streptococcus sobrinus, possessing insoluble glucan synthesis abilities. The MICs of biofilm formation by these bacteria ranged from 1/8 to 2× MIC. Furthermore, the inhibition effects of BGA against the coaggregation of Porphyromonas gingivalis and Streptococcus gordonii were evaluated. BGA at 32 or 64 μg/mL inhibited the coaggregation of these bacteria after a 30 min incubation. Lastly, the inhibition effects of BGA against human supragingival plaque bacteria were evaluated. Human supragingival plaque samples were obtained from 12 healthy donors. The inhibition effects of BGA against biofilm formation by these plaque bacteria were evaluated. Of 12 samples, the biofilm formation by 11 was significantly attenuated by 128-256 μg/mL of BGA. The number of colony forming units in these biofilms was also significantly attenuated. In conclusion, it was revealed that BGA inhibits the growth and biofilm formation of bacteria, furthermore, the same effect was confirmed with supragingival plaque bacteria. BGA is a good candidate for a natural agent that prevents the outbreak and progression of periodontal disease because it suppresses not only the growth and biofilm formation of bacteria, but also the coaggregation of P. gingivalis with plaque bacteria.
Volume 65(9)
Pages 343-351
Published 2021-9-1
DOI 10.1111/1348-0421.12884
PMID 33860563
MeSH Biofilms Glycyrrhetinic Acid* / pharmacology Humans Streptococcus gordonii Streptococcus mutans Streptococcus sobrinus
IF 1.566
General Microbes JCM 5707, JCM 8528, JCM 6328