Reference - Detail
| RRC ID | 77236 |
|---|---|
| Author | Kurata N, Shishido T, Muraoka M, Tanaka T, Ogino C, Fukuda H, Kondo A. |
| Title | Specific protein delivery to target cells by antibody-displaying bionanocapsules. |
| Journal | J Biochem |
| Abstract |
Bionanocapsules (BNCs) are nanoparticles with a high biocompatibility composed of the L protein of the hepatitis B virus surface antigen. BNC can deliver bioactive molecules to hepatocytes efficiently and specifically. However, delivery is limited to hepatocytes and incorporation of proteins into BNC is quite troublesome. Here, in order to alter the specificity of BNC and to achieve efficient protein delivery, we developed engineered BNC displaying the ZZ domain of protein A and incorporating enhanced green fluorescent protein (EGFP) inside the particles using an insect cell expression system. The ZZ domain displayed on the surface of BNC binds to anti-epidermal growth factor receptor (EGFR) antibodies, allowing specific delivery of EGFP to HeLa cells. The engineered BNCs are a promising and powerful tool for efficient and cell-specific protein delivery. |
| Volume | 144(6) |
| Pages | 701-7 |
| Published | 2008-12-1 |
| DOI | 10.1093/jb/mvn131 |
| PII | mvn131 |
| PMID | 18838435 |
| MeSH | Antibodies / chemistry Antibodies / metabolism Drug Carriers / chemistry* Drug Delivery Systems / methods ErbB Receptors / chemistry ErbB Receptors / metabolism Genetic Vectors / genetics Genetic Vectors / metabolism Green Fluorescent Proteins / chemistry Green Fluorescent Proteins / metabolism HeLa Cells Humans Nanoparticles / chemistry* Protein Engineering / methods Proteins / administration & dosage* |
| IF | 2.476 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) Hep G2 |