論文 - 詳細
| RRC ID | 77283 |
|---|---|
| 著者 | D'Elia KP, Hameedy H, Goldblatt D, Frazel P, Kriese M, Zhu Y, Hamling KR, Kawakami K, Liddelow SA, Schoppik D, Dasen JS. |
| タイトル | Determinants of motor neuron functional subtypes important for locomotor speed. |
| ジャーナル | Cell Rep |
| Abstract |
Locomotion requires precise control of the strength and speed of muscle contraction and is achieved by recruiting functionally distinct subtypes of motor neurons (MNs). MNs are essential to movement and differentially susceptible in disease, but little is known about how MNs acquire functional subtype-specific features during development. Using single-cell RNA profiling in embryonic and larval zebrafish, we identify novel and conserved molecular signatures for MN functional subtypes and identify genes expressed in both early post-mitotic and mature MNs. Assessing MN development in genetic mutants, we define a molecular program essential for MN functional subtype specification. Two evolutionarily conserved transcription factors, Prdm16 and Mecom, are both functional subtype-specific determinants integral for fast MN development. Loss of prdm16 or mecom causes fast MNs to develop transcriptional profiles and innervation similar to slow MNs. These results reveal the molecular diversity of vertebrate axial MNs and demonstrate that functional subtypes are specified through intrinsic transcriptional codes. |
| 巻・号 | 42(9) |
| ページ | 113049 |
| 公開日 | 2023-9-26 |
| DOI | 10.1016/j.celrep.2023.113049 |
| PII | S2211-1247(23)01060-4 |
| PMID | 37676768 |
| PMC | PMC10600875 |
| MeSH | Animals Locomotion Motor Neurons / physiology Spinal Cord* Transcription Factors / genetics Zebrafish* |
| IF | 8.109 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 24 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | gSAIzGFFM1116A, UAS:GFP |