| RRC ID |
77311
|
| 著者 |
Shimoda S, Ichihara H, Matsumoto Y, Ueoka R.
|
| タイトル |
Chemotherapy with hybrid liposomes for human breast tumors along with apoptosis in vivo.
|
| ジャーナル |
Int J Pharm
|
| Abstract |
Hybrid liposomes (HL-n) can be prepared by dissolving both vesicular and micellar molecules in buffer solution with ultrasonication. A clear solution of HL-n composed of 95 mol% dimyristoylphosphatidylcholine (DMPC) and 5 mol% polyoxyethylenedodecyl ether (C(12)(EO)(n), n=21 and 25) having hydrodynamic diameter of 100 nm could be kept over 1 month on the basis of dynamic light scattering measurements was obtained. (1) Increases of fusion and accumulation of HL-n including NBDPC as a fluorescence probe into human breast tumor (MDA-MB-453) cell membranes were observed. (2) Reduction of mitochondrial membrane potential and activation of caspase-8, caspase-9, and caspase-3 were observed, indicating that apoptotic signal by HL-n should pass through mitochondria and these caspases. (3) Remarkable reduction of tumor volume in a xenograft mice model intravenously treated with HL-n without drugs after the subcutaneously inoculation of MDA-MB-453 cells was verified in vivo. Induction of apoptosis in tumor of xenograft mice treated with HL-n was observed in micrographs on the basis of TUNEL method. It was noteworthy that the therapeutic effects of HL-n along with apoptosis were obtained for xenograft mice model of human breast tumor in vivo.
|
| 巻・号 |
372(1-2)
|
| ページ |
162-8
|
| 公開日 |
2009-5-8
|
| DOI |
10.1016/j.ijpharm.2009.01.011
|
| PII |
S0378-5173(09)00031-3
|
| PMID |
19429276
|
| MeSH |
Animals
Antineoplastic Agents / chemical synthesis
Antineoplastic Agents / pharmacology
Antineoplastic Agents / therapeutic use
Apoptosis / drug effects*
Apoptosis / physiology
Breast Neoplasms / drug therapy*
Breast Neoplasms / metabolism
Breast Neoplasms / pathology
Cell Line, Tumor
Drug Screening Assays, Antitumor / methods
Female
Humans
Liposomes / chemical synthesis
Liposomes / pharmacology
Liposomes / therapeutic use*
Mice
Mice, Inbred BALB C
Mice, Nude
Mitochondria / drug effects
Mitochondria / metabolism
|
| IF |
4.845
|
| リソース情報 |
| ヒト・動物細胞 |
MDA-MB-453(RCB1192) |
