論文 - 詳細
| RRC ID | 77320 |
|---|---|
| 著者 | Hirakawa H, Kodama T, Takumi-Kobayashi A, Honda T, Yamaguchi A. |
| タイトル | Secreted indole serves as a signal for expression of type III secretion system translocators in enterohaemorrhagic Escherichia coli O157:H7. |
| ジャーナル | Microbiology (Reading) |
| Abstract |
Indole is produced by tryptophanase during growth of enteric bacteria and accumulates in the culture medium. The physiological role of indole production is poorly understood. We discovered that enterohaemorrhagic Escherichia coli (EHEC) O157:H7 with a tnaA deletion has decreased secretion of EspA and EspB via the type III secretion system and as a result there is reduced formation of attaching and effacing (A/E) lesions in HeLa cells. Addition of indole restored and enhanced secretion of EspA and EspB and formation of A/E lesions by the tnaA deletion mutant EHEC. Indole addition moderately increased the promoter activity of LEE4 genes, including espA and espB, in the locus of enterocyte effacement. Thus in EHEC indole can serve to signal EspA and EspB expression and secretion and stimulate the ability of EHEC to form A/E lesions on human cells. |
| 巻・号 | 155(Pt 2) |
| ページ | 541-550 |
| 公開日 | 2009-2-1 |
| DOI | 10.1099/mic.0.020420-0 |
| PMID | 19202102 |
| MeSH | Bacterial Outer Membrane Proteins / genetics Bacterial Outer Membrane Proteins / metabolism* Escherichia coli Infections / microbiology* Escherichia coli O157 / genetics Escherichia coli O157 / metabolism* Escherichia coli O157 / pathogenicity Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial* HeLa Cells Humans Indoles / metabolism* Phosphoproteins / genetics Phosphoproteins / metabolism Promoter Regions, Genetic Protein Transport Sequence Deletion Tryptophanase / genetics Tryptophanase / metabolism |
| IF | 2.138 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |