論文 - 詳細
| RRC ID | 77362 |
|---|---|
| 著者 | Tsurushima H, Marushima A, Suzuki K, Oyane A, Sogo Y, Nakamura K, Matsumura A, Ito A. |
| タイトル | Enhanced bone formation using hydroxyapatite ceramic coated with fibroblast growth factor-2. |
| ジャーナル | Acta Biomater |
| Abstract |
Our objective was to develop a bone substitute coated with fibroblast growth factor-2 (FGF-2) that subsequently releases FGF-2. We investigated the use of our system of bone substitutes to induce bone formation. Hydroxyapatite ceramic buttons (HAP-CBs) were coated with FGF-2 by precipitation in supersaturated calcium phosphate solution. HAP-CBs were coated with high or low doses of FGF-2, denoted as FGF-H and FGF-L. The release of FGF-2 from FGF-H and FGF-L was evaluated using its release profile and bioactivity. The efficacy of the subsequent bone formation was quantified using rats with round-shaped bone defects (5mm in diameter) of the right parietal bone. Group 1 was treated only with HAP-CBs, group 2 with HAP-CBs and drops of FGF-2 solution, group 3 with FGF-L and group 4 with FGF-H. To detect the release of FGF-2 in vivo, the expression of bone morphogenic protein-2 (BMP-2) was measured in the defective bone tissue. FGF-2 was released in vitro from FGF-H and FGF-L, and maintained its bioactivity. Rats treated with FGF-L showed better bone formation than rats from the other groups. BMP-2 expression was detected in the defective bone tissues of group 3 at 14 days, which might indicate in vivo FGF-2 release during this period. A specific FGF-2 concentration may be needed for bone formation, and our system can release FGF-2 at adequate concentrations to induce bone formation. |
| 巻・号 | 6(7) |
| ページ | 2751-9 |
| 公開日 | 2010-7-1 |
| DOI | 10.1016/j.actbio.2009.12.045 |
| PII | S1742-7061(09)00586-8 |
| PMID | 20045091 |
| MeSH | Alkaline Phosphatase / metabolism Animals Base Sequence Bone Development* Bone Morphogenetic Protein 2 / metabolism Bone and Bones / enzymology Bone and Bones / metabolism Ceramics* DNA Primers Fibroblast Growth Factor 2* Hydroxyapatites* Microscopy, Electron, Scanning Osteocalcin / metabolism Polymerase Chain Reaction Rats |
| IF | 7.242 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MG-63(RCB1890) |