| RRC ID |
77362
|
| 著者 |
Tsurushima H, Marushima A, Suzuki K, Oyane A, Sogo Y, Nakamura K, Matsumura A, Ito A.
|
| タイトル |
Enhanced bone formation using hydroxyapatite ceramic coated with fibroblast growth factor-2.
|
| ジャーナル |
Acta Biomater
|
| Abstract |
Our objective was to develop a bone substitute coated with fibroblast growth factor-2 (FGF-2) that subsequently releases FGF-2. We investigated the use of our system of bone substitutes to induce bone formation. Hydroxyapatite ceramic buttons (HAP-CBs) were coated with FGF-2 by precipitation in supersaturated calcium phosphate solution. HAP-CBs were coated with high or low doses of FGF-2, denoted as FGF-H and FGF-L. The release of FGF-2 from FGF-H and FGF-L was evaluated using its release profile and bioactivity. The efficacy of the subsequent bone formation was quantified using rats with round-shaped bone defects (5mm in diameter) of the right parietal bone. Group 1 was treated only with HAP-CBs, group 2 with HAP-CBs and drops of FGF-2 solution, group 3 with FGF-L and group 4 with FGF-H. To detect the release of FGF-2 in vivo, the expression of bone morphogenic protein-2 (BMP-2) was measured in the defective bone tissue. FGF-2 was released in vitro from FGF-H and FGF-L, and maintained its bioactivity. Rats treated with FGF-L showed better bone formation than rats from the other groups. BMP-2 expression was detected in the defective bone tissues of group 3 at 14 days, which might indicate in vivo FGF-2 release during this period. A specific FGF-2 concentration may be needed for bone formation, and our system can release FGF-2 at adequate concentrations to induce bone formation.
|
| 巻・号 |
6(7)
|
| ページ |
2751-9
|
| 公開日 |
2010-7-1
|
| DOI |
10.1016/j.actbio.2009.12.045
|
| PII |
S1742-7061(09)00586-8
|
| PMID |
20045091
|
| MeSH |
Alkaline Phosphatase / metabolism
Animals
Base Sequence
Bone Development*
Bone Morphogenetic Protein 2 / metabolism
Bone and Bones / enzymology
Bone and Bones / metabolism
Ceramics*
DNA Primers
Fibroblast Growth Factor 2*
Hydroxyapatites*
Microscopy, Electron, Scanning
Osteocalcin / metabolism
Polymerase Chain Reaction
Rats
|
| IF |
7.242
|
| リソース情報 |
| ヒト・動物細胞 |
MG-63(RCB1890) |
