| RRC ID |
77412
|
| 著者 |
Nagae M, Yamada K, Enomoto Y, Kometani M, Tsuchida H, Panthee A, Nonogaki M, Matsunaga N, Takizawa M, Matsuzaki S, Hirabayashi M, Inoue N, Tsukamura H, Uenoyama Y.
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| タイトル |
Conditional Oprk1-dependent Kiss1 deletion in kisspeptin neurons caused estrogen-dependent LH pulse disruption and LH surge attenuation in female rats.
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| ジャーナル |
Sci Rep
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| Abstract |
The gonadotropin-releasing hormone (GnRH) pulse and surge are considered to be generated by arcuate kisspeptin/neurokinin B/dynorphin A (KNDy) neurons and anteroventral periventricular nucleus (AVPV) kisspeptin neurons, respectively, in female rodents. The majority of KNDy and AVPV kisspeptin neurons express κ-opioid receptors (KORs, encoded by Oprk1) in female rodents. Thus, this study aimed to investigate the effect of a conditional Oprk1-dependent Kiss1 deletion in kisspeptin neurons on the luteinizing hormone (LH) pulse/surge and fertility using Kiss1-floxed/Oprk1-Cre rats, in which Kiss1 was deleted in cells expressing or once expressed the Oprk1/Cre. The Kiss1-floxed/Oprk1-Cre female rats, with Kiss1 deleted in a majority of KNDy neurons, showed normal puberty while having a one-day longer estrous cycle and fewer pups than Kiss1-floxed controls. Notably, ovariectomized (OVX) Kiss1-floxed/Oprk1-Cre rats showed profound disruption of LH pulses in the presence of a diestrous level of estrogen but showed apparent LH pulses without estrogen treatment. Furthermore, Kiss1-floxed/Oprk1-Cre rats, with Kiss1 deleted in approximately half of AVPV kisspeptin neurons, showed a lower peak of the estrogen-induced LH surge than controls. These results suggest that arcuate and AVPV kisspeptin neurons expressing or having expressed Oprk1 have a role in maintaining normal GnRH pulse and surge generation, the normal length of the estrous cycle, and the normal offspring number in female rats.
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| 巻・号 |
13(1)
|
| ページ |
20495
|
| 公開日 |
2023-11-22
|
| DOI |
10.1038/s41598-023-47222-5
|
| PII |
10.1038/s41598-023-47222-5
|
| PMID |
37993510
|
| PMC |
PMC10665460
|
| MeSH |
Animals
Arcuate Nucleus of Hypothalamus / metabolism
Dynorphins / metabolism
Estrogens / pharmacology
Female
Gonadotropin-Releasing Hormone / metabolism
Kisspeptins* / metabolism
Luteinizing Hormone* / pharmacology
Neurokinin B / genetics
Neurokinin B / metabolism
Neurons / metabolism
Rats
|
| IF |
3.998
|
| リソース情報 |
| ラット |
LE-Tg(Gt(ROSA)26Sor-CAG-tdTomato)24Jfhy (StrainID=1139) |
