| RRC ID |
77446
|
| 著者 |
Isono K, Nakamura K, Hanada K, Shirai K, Ueki M, Tanaka K, Tsuchimatsu T, Iuchi S, Kobayashi M, Yotsui I, Sakata Y, Taji T.
|
| タイトル |
LHT1/MAC7 contributes to proper alternative splicing under long-term heat stress and mediates variation in the heat tolerance of Arabidopsis.
|
| ジャーナル |
PNAS Nexus
|
| Abstract |
Natural genetic variation has facilitated the identification of genes underlying complex traits such as stress tolerances. We here evaluated the long-term (L-) heat tolerance (37°C for 5 days) of 174 Arabidopsis thaliana accessions and short-term (S-) heat tolerance (42°C, 50 min) of 88 accessions and found extensive variation, respectively. Interestingly, L-heat-tolerant accessions are not necessarily S-heat tolerant, suggesting that the tolerance mechanisms are different. To elucidate the mechanisms underlying the variation, we performed a chromosomal mapping using the F2 progeny of a cross between Ms-0 (a hypersensitive accession) and Col-0 (a tolerant accession) and found a single locus responsible for the difference in L-heat tolerance between them, which we named Long-term Heat Tolerance 1 (LHT1). LHT1 is identical to MAC7, which encodes a putative RNA helicase involved in mRNA splicing as a component of the MOS4 complex. We found one amino acid deletion in LHT1 of Ms-0 that causes a loss of function. Arabidopsis mutants of other core components of the MOS4 complex-mos4-2, cdc5-1, mac3a mac3b, and prl1 prl2-also showed hypersensitivity to L-heat stress, suggesting that the MOS4 complex plays an important role in L-heat stress responses. L-heat stress induced mRNA processing-related genes and compromised alternative splicing. Loss of LHT1 function caused genome-wide detrimental splicing events, which are thought to produce nonfunctional mRNAs that include retained introns under L-heat stress. These findings suggest that maintaining proper alternative splicing under L-heat stress is important in the heat tolerance of A. thaliana.
|
| 巻・号 |
2(11)
|
| ページ |
pgad348
|
| 公開日 |
2023-11-1
|
| DOI |
10.1093/pnasnexus/pgad348
|
| PII |
pgad348
|
| PMID |
38024403
|
| PMC |
PMC10644991
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
sja15700 |
