Reference - Detail
| RRC ID | 77460 |
|---|---|
| Author | Yuko Watanabe, Eri Katsumura, Tatsuki Domon, Yuta Ishikawa, Rina Oguri, Minami Takashima, Qi Meng, Masato Kinoshita, Hisashi Hashimoto, Kiyotaka Hitomi |
| Title | Establishment of transgenic epithelium-specific Cre-recombinase driving medaka (Oryzias latipes) by homology repair mediated knock-in |
| Journal | Bioscience, Biotechnology, and Biochemistry |
| Abstract |
ABSTRACT
Deletion of gene expression in the target tissues and cells is an effective strategy for elucidating the physiological functions of the protein of interest. For tissue-specific and/or inducible gene deletion, the Cre-loxP system has been widely used in various model organisms including medaka (Oryzias latipes). The epithelium is the key tissue, locating at the outermost area and playing a role in barrier to external stimuli. Despite a large genetic toolbox developed in medaka, there is no available Cre-driver line that works in an epithelium-specific manner. Here, we established epithelium-specific Cre-driver lines in medaka using a homology-directed repair mediated knock-in approach with CRISPR/Cas9, targeting each of periplakin and keratin genes. We show that Cre-recombinase is expressed exclusively in the epithelium in the knock-in lines and that it efficiently and specifically induces recombination in the tissues. These Cre-driver lines are useful for studying the functions of proteins expressed in the epithelium. |
| Volume | 87 |
| Pages | 1285-1294 |
| Published | 2023-10-25 |
| DOI | 10.1093/bbb/zbad116 |
| PMID | 37607777 |
| MeSH | Animals Animals, Genetically Modified Integrases / genetics Integrases / metabolism Oryzias* / genetics |
| IF | 1.516 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Medaka | OK-Cab (MT830) d-rR-Tg(beta-actin-loxP-DsRed2-loxP-GFP) (TG861) |