Reference - Detail
| RRC ID | 77468 |
|---|---|
| Author | Tada R, Higashidate T, Amano T, Ishikawa S, Yokoyama C, Kobari S, Nara S, Ishida K, Kawaguchi A, Ochi H, Ogino H, Yakushiji-Kaminatsui N, Sakamoto J, Kamei Y, Tamura K, Yokoyama H. |
| Title | The shh limb enhancer is activated in patterned limb regeneration but not in hypomorphic limb regeneration in Xenopus laevis. |
| Journal | Dev Biol |
| Abstract |
Xenopus young tadpoles regenerate a limb with the anteroposterior (AP) pattern, but metamorphosed froglets regenerate a hypomorphic limb after amputation. The key gene for AP patterning, shh, is expressed in a regenerating limb of the tadpole but not in that of the froglet. Genomic DNA in the shh limb-specific enhancer, MFCS1 (ZRS), is hypermethylated in froglets but hypomethylated in tadpoles: shh expression may be controlled by epigenetic regulation of MFCS1. Is MFCS1 specifically activated for regenerating the AP-patterned limb? We generated transgenic Xenopus laevis lines that visualize the MFCS1 enhancer activity with a GFP reporter. The transgenic tadpoles showed GFP expression in hoxd13-and shh-expressing domains of developing and regenerating limbs, whereas the froglets showed no GFP expression in the regenerating limbs despite having hoxd13 expression. Genome sequence analysis and co-transfection assays using cultured cells revealed that Hoxd13 can activate Xenopus MFCS1. These results suggest that MFCS1 activation correlates with regeneration of AP-patterned limbs and that re-activation of epigenetically inactivated MFCS1 would be crucial to confer the ability to non-regenerative animals for regenerating a properly patterned limb. |
| Volume | 500 |
| Pages | 22-30 |
| Published | 2023-8-1 |
| DOI | 10.1016/j.ydbio.2023.05.009 |
| PII | S0012-1606(23)00093-3 |
| PMID | 37247832 |
| MeSH | Animals Animals, Genetically Modified Epigenesis, Genetic* Extremities* / physiology Transcription Factors / genetics Xenopus laevis / genetics |
| IF | 2.896 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 19 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | MFCS1-luciferace (RDB19989) MFCS1-pshh-luciferace (RDB19990) pCMV-Hoxd13 (RDB19991) pCMV-Fgf2 (RDB19992) pCMV-Fgf4 (RDB19993) pCMV-Fgf8 (RDB19994) |