論文 - 詳細
| RRC ID | 77483 |
|---|---|
| 著者 | Hagiwara H, Wako H, Nakata K, Aida R. |
| タイトル | Genistein Induces Antiproliferative Activity and Apoptosis in Human Osteosarcoma Saos-2 Cells. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Genistein (4', 5, 7-trihydroxyisoflavone) and daidzein (4', 7-dihydroxyisoflavone) are isoflavones derived from soybean and have anti-cancer effects in various cells. However, the effects of genistein and daidzein on the human osteosarcoma cell line Saos-2 has not been investigated before. MATERIALS AND METHODS:Human osteosarcoma Saos-2 cells were treated with genistein for 24 and 48 hours. Cytotoxicity and apoptosis were measured. RESULTS:Genistein significantly inhibited proliferation of Saos-2 cells stronger than daidzein in a dose-dependent manner (0 to 80 μM). Genistein also significantly suppressed Saos-2 cell viability in a dose-dependent manner (0 to 100 μM). In contrast, daidzein did not affect Saos-2 cell viability. Real-time PCR revealed that genistein caused G1-arrest by increasing the expression of p21 and p27 mRNAs in Saos-2 cells. In addition, genistein induced apoptosis through the up-regulation of effector caspase-3/7 activity in Saos-2 cells. Genistein also enhanced initiator caspase-9 and TNF-α mRNA expression in cells. CONCLUSION:Genistein may inhibit proliferation through the up-regulation of p21 and p27 and viability by inducing apoptosis in Saos-2 cells. |
| 巻・号 | 43(12) |
| ページ | 5387-5392 |
| 公開日 | 2023-12-1 |
| DOI | 10.21873/anticanres.16742 |
| PII | 43/12/5387 |
| PMID | 38030209 |
| MeSH | Apoptosis Bone Neoplasms* / drug therapy Cell Line, Tumor Cell Proliferation Genistein / pharmacology Humans Isoflavones* / pharmacology Osteosarcoma* / drug therapy Osteosarcoma* / metabolism |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | Saos-2(RCB0428) |