RRC ID |
77518
|
Author |
Kouroku Y, Fujita E, Jimbo A, Mukasa T, Tsuru T, Momoi MY, Momoi T.
|
Title |
Localization of active form of caspase-8 in mouse L929 cells induced by TNF treatment and polyglutamine aggregates.
|
Journal |
Biochem Biophys Res Commun
|
Abstract |
The relation between activation of caspase-8 and polyglutamine aggregates has been focused. We prepared an antiserum (anti-m8D387) that recognizes the active form but not the proform of mouse caspase-8. We used immunostaining with anti-m8D387 antiserum to compare the localizations of activated mcaspase-8 in L929 (clone 1422) cells induced by TNF and polyglutamine aggregates. Anti-m8D387 was positive throughout cytoplasm of the TUNEL-positive cells induced by TNF treatment, whereas the anti-m8D387 reactivity was not positive throughout cytoplasm of the cells expressing polyglutamine but was restricted to polyglutamine aggregates. In contrast with TNF-treated cells, cells expressing anti-m8D387-positive cytoplasmic polyglutamine aggregates did not undergo TUNEL-positive apoptosis. Thus activated caspase-8 associated with polyglutamine aggregates alone was not sufficient to induce TUNEL-positive apoptosis of L929 (clone 1422) cells. The distribution of activated caspase-8 associated with polyglutamine aggregates may be essential for the polyglutamine-mediated cell death or downstream of caspase-8 may be different in the TNF-treated cells and cells expressing polyglutamine.
|
Volume |
270(3)
|
Pages |
972-7
|
Published |
2000-4-21
|
DOI |
10.1006/bbrc.2000.2463
|
PII |
S0006-291X(00)92463-X
|
PMID |
10772935
|
MeSH |
Animals
COS Cells
Caspase 8
Caspase 9
Caspases / analysis
Caspases / metabolism*
Fibrosarcoma
Green Fluorescent Proteins
Luminescent Proteins / analysis
Mice
Peptides / analysis
Peptides / metabolism*
Recombinant Fusion Proteins / analysis
Recombinant Fusion Proteins / metabolism
Transfection
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha / pharmacology*
|
IF |
2.985
|
Resource |
Human and Animal Cells |
L929 |