RRC ID 77537
Author Minegishi M, Kuchimaru T, Nishikawa K, Isagawa T, Iwano S, Iida K, Hara H, Miura S, Sato M, Watanabe S, Shiomi A, Mabuchi Y, Hamana H, Kishi H, Sato T, Sawaki D, Sato S, Hanazono Y, Suzuki A, Kohro T, Kadonosono T, Shimogori T, Miyawaki A, Takeda N, Shintaku H, Kizaka-Kondoh S, Nishimura S.
Title Secretory GFP reconstitution labeling of neighboring cells interrogates cell-cell interactions in metastatic niches.
Journal Nat Commun
Abstract Cancer cells inevitably interact with neighboring host tissue-resident cells during the process of metastatic colonization, establishing a metastatic niche to fuel their survival, growth, and invasion. However, the underlying mechanisms in the metastatic niche are yet to be fully elucidated owing to the lack of methodologies for comprehensively studying the mechanisms of cell-cell interactions in the niche. Here, we improve a split green fluorescent protein (GFP)-based genetically encoded system to develop secretory glycosylphosphatidylinositol-anchored reconstitution-activated proteins to highlight intercellular connections (sGRAPHIC) for efficient fluorescent labeling of tissue-resident cells that neighbor on and putatively interact with cancer cells in deep tissues. The sGRAPHIC system enables the isolation of metastatic niche-associated tissue-resident cells for their characterization using a single-cell RNA sequencing platform. We use this sGRAPHIC-leveraged transcriptomic platform to uncover gene expression patterns in metastatic niche-associated hepatocytes in a murine model of liver metastasis. Among the marker genes of metastatic niche-associated hepatocytes, we identify Lgals3, encoding galectin-3, as a potential pro-metastatic factor that accelerates metastatic growth and invasion.
Volume 14(1)
Pages 8031
Published 2023-12-5
DOI 10.1038/s41467-023-43855-2
PII 10.1038/s41467-023-43855-2
PMID 38052804
PMC PMC10697979
MeSH Animals Cell Communication Extracellular Matrix / metabolism Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Hepatocytes / metabolism Humans Liver Neoplasms* / metabolism Mice
IF 12.121
Human and Animal Cells KUSA-A1(RCB2081)
DNA material CSII-CMV-MCS (RDB04377) pCMV-VSV-G-RSV-Rev (RDB04393)