RRC ID 77584
Author Hatakeyama Y, Saito N, Mii Y, Takada R, Shinozuka T, Takemoto T, Naoki H, Takada S.
Title Intercellular exchange of Wnt ligands reduces cell population heterogeneity during embryogenesis.
Journal Nat Commun
Abstract Wnt signaling is required to maintain bipotent progenitors for neural and paraxial mesoderm cells, the neuromesodermal progenitor (NMP) cells that reside in the epiblast and tailbud. Since epiblast/tailbud cells receive Wnt ligands produced by one another, this exchange may average out the heterogeneity of Wnt signaling levels among these cells. Here, we examined this possibility by replacing endogenous Wnt3a with a receptor-fused form that activates signaling in producing cells, but not in neighboring cells. Mutant mouse embryos show a unique phenotype in which maintenance of many NMP cells is impaired, although some cells persist for long periods. The epiblast cell population of these embryos increases heterogeneity in Wnt signaling levels as embryogenesis progresses and are sensitive to retinoic acid, an endogenous antagonist of NMP maintenance. Thus, mutual intercellular exchange of Wnt ligands in the epiblast cell population reduces heterogeneity and achieves robustness to environmental stress.
Volume 14(1)
Pages 1924
Published 2023-4-6
DOI 10.1038/s41467-023-37350-x
PII 10.1038/s41467-023-37350-x
PMID 37024462
PMC PMC10079677
MeSH Animals Cell Differentiation / genetics Embryonic Development / genetics Gene Expression Regulation, Developmental Germ Layers* Mesoderm* Mice Wnt Signaling Pathway / physiology
IF 12.121
Mice RBRC00113