論文 - 詳細
| RRC ID | 77621 |
|---|---|
| 著者 | Taketomi A, Shirabe K, Muto J, Yoshiya S, Motomura T, Mano Y, Ikegami T, Yoshizumi T, Sugio K, Maehara Y. |
| タイトル | A rare point mutation in the Ras oncogene in hepatocellular carcinoma. |
| ジャーナル | Surg Today |
| Abstract |
PURPOSE:The Ras gene is one of the oncogenes most frequently detected in human cancers, and codes for three proteins (K-, N-, and H-Ras). The aim of this study was to examine the mutations in codons 12, 13 and 61 of the three Ras genes in cases of human hepatocellular carcinoma (HCC). METHODS:Paired samples of HCC and corresponding non-malignant liver tissue were collected from 61 patients who underwent hepatectomy. A dot-blot analysis was used to analyze the products of the polymerase chain reaction (PCR) amplification of codons 12, 13, and 61 of K-, N- and H-Ras for mutations. RESULTS:Only one mutation (K-Ras codon 13; Gly to Asp) was detected among the 61 patients. Interestingly, this patient had a medical history of surgery for both gastric cancer and right lung cancer. No mutations were found in codons 12 and 61 of K-Ras or codons 12, 13 and 61 of the N-Ras and H-Ras genes in any of the HCCs or corresponding non-malignant tissues. CONCLUSIONS:These findings indicated that the activation of Ras proto-oncogenes by mutations in codons 12, 13, and 61 does not play a major role in hepatocellular carcinogenesis. |
| 巻・号 | 43(3) |
| ページ | 289-92 |
| 公開日 | 2013-3-1 |
| DOI | 10.1007/s00595-012-0462-8 |
| PMID | 23266750 |
| MeSH | Adult Aged Carcinoma, Hepatocellular / genetics* Codon DNA, Neoplasm / genetics* Female Genes, ras / genetics* Humans Liver Neoplasms / genetics* Male Middle Aged Point Mutation* Polymerase Chain Reaction Retrospective Studies |
| IF | 1.878 |
| リソース情報 | |
| ヒト・動物細胞 | HuH-7(RCB1942) |