Reference - Detail
| RRC ID | 77743 |
|---|---|
| Author | Kawai K, Sasaki T, Saijo-Kurita K, Akaza H, Koiso K, Ohno T. |
| Title | Additive effects of antitumor drugs and lymphokine-activated killer cell cytotoxic activity in tumor cell killing determined by lactate-dehydrogenase-release assay. |
| Journal | Cancer Immunol Immunother |
| Abstract |
The effect of pretreatment with antitumor drugs on lymphokine-activated killer (LAK) cell cytotoxic activity, determined by lactate-dehydrogenase(LDH)-release assay, was investigated. LAK cells were induced by incubating peripheral blood lymphocytes of healthy donors in medium containing interleukin-2 (IL-2) and monoclonal anti-CD3 antibody for 6-7 days. A human lung squamous carcinoma cell line, SQ-5, was used as an adherent target. After 24 h exposure of the target cells to cisplatin, doxorubicin, or mitomycin C, the drugs were washed off and LAK cells were added at an E/T ratio of 5. During further incubation for 48 h, LDH release from cisplatin- or doxorubicin-pretreated target cells was markedly higher than that from non-pretreated target cells. The combination of cisplatin and LAK cells has an additive cytotoxic effect and that of mitomycin C and LAK cells does not; there may also be an additive effect late in the toxicity mechanism between doxorubicin and LAK cells. |
| Volume | 35(4) |
| Pages | 225-9 |
| Published | 1992-1-1 |
| DOI | 10.1007/BF01789327 |
| PMID | 1511457 |
| MeSH | Antineoplastic Agents / pharmacology* Carcinoma, Squamous Cell / enzymology Carcinoma, Squamous Cell / pathology Carcinoma, Squamous Cell / therapy Cell Death / drug effects Cell Survival / drug effects Clinical Enzyme Tests / methods Combined Modality Therapy Cytotoxicity, Immunologic Drug Synergism Humans Immunotherapy, Adoptive* Killer Cells, Lymphokine-Activated / drug effects Killer Cells, Lymphokine-Activated / immunology* L-Lactate Dehydrogenase / analysis L-Lactate Dehydrogenase / metabolism* Lung Neoplasms / enzymology Lung Neoplasms / pathology Lung Neoplasms / therapy Time Factors Tumor Cells, Cultured / enzymology |
| IF | 5.442 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | SQ-5(RCB0110) |