RRC ID 77832
Author Okamoto M, Miura A, Ito R, Kamada T, Mizukami Y, Kawamoto K.
Title G-protein-coupled estrogen receptor prevents nuclear factor-kappa B promoter activation by Helicobacter pylori cytotoxin-associated gene A in gastric cancer cells.
Journal J Vet Med Sci
Abstract Helicobacter pylori is a well-known pathogen that causes chronic gastritis, leading to the development of gastric cancer. This bacterium has also been detected in dogs, and symptoms similar to those in humans have been reported. The cytotoxin-associated gene A (CagA) is involved in pathogenesis through aberrant activation of host signal transduction, including the nuclear factor-kappa B (NF-κB) pathway. We have previously shown the anti-inflammatory effect of the G-protein-coupled estrogen receptor (GPER) via inhibiting of NF-κB activation in several cells. Therefore, here, we investigated the effect of GPER on CagA-mediated NF-κB promoter activity and showed that CagA overexpression in gastric cancer cells activated the NF-κB reporter and induced interleukin 8 (il-8) expression, both of which were inhibited by the GPER agonist.
Volume 85(12)
Pages 1348-1354
Published 2023-12-27
DOI 10.1292/jvms.23-0054
PMID 37952974
MeSH Animals Cytotoxins / metabolism Cytotoxins / pharmacology Dog Diseases* / metabolism Dogs GTP-Binding Proteins / metabolism GTP-Binding Proteins / pharmacology Gastric Mucosa / metabolism Helicobacter Infections* / metabolism Helicobacter Infections* / veterinary Helicobacter pylori* / genetics Helicobacter pylori* / metabolism Humans Interleukin-8 / genetics NF-kappa B / metabolism Receptors, Estrogen / genetics Receptors, Estrogen / metabolism Stomach Neoplasms* / genetics Stomach Neoplasms* / metabolism Stomach Neoplasms* / veterinary
IF 1.049
DNA material Genomic DNA of Helicobacter pylori JCM 12093 T (JGD07707)