論文 - 詳細
| RRC ID | 77845 |
|---|---|
| 著者 | Satoh JI, Kuroda Y. |
| タイトル | Ubiquitin C-terminal hydrolase-L1 (PGP9.5) expression in human neural cell lines following induction of neuronal differentiation and exposure to cytokines, neurotrophic factors or heat stress. |
| ジャーナル | Neuropathol Appl Neurobiol |
| Abstract |
Dysfunction of the ubiquitin-dependent proteolytic pathway contributes to progressive accumulation of ubiquitinated protein inclusions in neurodegenerative disorders, such as Parkinson's disease (PD). Ubiquitin C-terminal hydrolase-L1 (UCH-L1), alternatively designated protein gene product 9.5 (PGP9.5), is a neural deubiquitinating enzyme which is identified as a principal constituent of Lewy bodies. To clarify the regulatory mechanism of UCH-L1 expression in human neural cells, we studied the constitutive, cytokine/neurotrophic factor-regulated, and heat stress-induced expression of UCH-L1 in cultured human neural cell lines by Western blot analysis. The constitutive expression of UCH-L1 was identified in SK-N-SH neuroblastoma cells, IMR-32 neuroblastoma cells, U-373MG astrocytoma cells, and NTera2 teratocarcinoma-derived differentiated neurones (NTera2-N). The levels of UCH-L1 expression were unaltered in these cell lines following treatment with TNF-alpha, IL-1beta, BDNF, GDNF, dibutyryl cyclic AMP, or phorbol 12-myristate 13-acetate, and remained unchanged by exposure to heat stress. In contrast, its levels were elevated substantially in NTera2 teratocarcinoma cells following retinoic acid-induced neuronal differentiation, accompanied with an increased expression of alpha-synuclein and synaptophysin. These results indicate that UCH-L1 is expressed constitutively in human neual cell lines, where it is upregulated following induction of neuronal differentiation, but unaffected by exposure to heat stress, cytokines, or growth/differentiation factors which are supposed to be invloved in the nigral neuronal death and survival in PD. |
| 巻・号 | 27(2) |
| ページ | 95-104 |
| 公開日 | 2001-4-1 |
| DOI | 10.1046/j.1365-2990.2001.00313.x |
| PII | nan313 |
| PMID | 11437990 |
| MeSH | Brain-Derived Neurotrophic Factor / pharmacology* Cell Death / drug effects Cell Death / physiology Cell Differentiation / drug effects Cell Differentiation / physiology Glial Cell Line-Derived Neurotrophic Factor Heat-Shock Response / physiology Humans Interleukin-1 / pharmacology* Lewy Bodies / metabolism Nerve Growth Factors* Nerve Tissue Proteins / pharmacology Neurons / cytology Neurons / enzymology* Teratocarcinoma Thiolester Hydrolases / analysis Thiolester Hydrolases / biosynthesis* Thiolester Hydrolases / metabolism Tumor Cells, Cultured Tumor Necrosis Factor-alpha / pharmacology* Ubiquitin Thiolesterase |
| IF | 7.5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | SK-N-SH(RCB0426) |