| RRC ID |
77848
|
| 著者 |
Yamada T, Maeda M, Nagai H, Salamin K, Chang Y-T, Guenova E, Feuermann M, Monod M.
|
| タイトル |
Two different types of tandem sequences mediate the overexpression of TinCYP51B in azole-resistant Trichophyton indotineae.
|
| ジャーナル |
Antimicrob Agents Chemother
|
| Abstract |
Trichophyton indotineae is an emerging dermatophyte that causes severe tinea corporis and tinea cruris. Numerous cases of terbinafine- and azole-recalcitrant T. indotineae-related dermatophytosis have been observed in India over the past decade, and cases are now being recorded worldwide. Whole genome sequencing of three azole-resistant strains revealed a variable number of repeats of a 2,404 base pair (bp) sequence encoding TinCYP51B in tandem specifically at the CYP51B locus position. However, many other resistant strains (itraconazole MIC ≥0.25 µg/mL; voriconazole MIC ≥0.25 µg/mL) did not contain such duplications. Whole-genome sequencing of three of these strains revealed a variable number of 7,374 bp tandem repeat blocks harboring TinCYP51B. Consequently, two types of T. indotineae azole-resistant strains were found to host TinCYP51B in tandem sequences (type I with 2,404 bp TinCYP51B blocks and type II with 7,374 bp TinCYP51B blocks). Using the CRISPR/Cas9 genome-editing tool, the copy number of TinCYP51B within the genome of types I and II strains was brought back to a single copy. The azole susceptibility of these modified strains was similar to that of strains without TinCYP51B duplication, showing that azole resistance in T. indotineae strains is mediated by one of two types of TinCYP51B amplification. Type II strains were prevalent among 32 resistant strains analyzed using a rapid and reliable PCR test.
|
| 巻・号 |
67(11)
|
| ページ |
e0093323
|
| 公開日 |
2023-11-15
|
| DOI |
10.1128/aac.00933-23
|
| PMID |
37823662
|
| PMC |
PMC10648874
|
| MeSH |
Antifungal Agents* / pharmacology
Arthrodermataceae*
Azoles / pharmacology
Drug Resistance, Fungal / genetics
Microbial Sensitivity Tests
Terbinafine / pharmacology
Trichophyton
|
| IF |
4.904
|
| リソース情報 |
| 病原真核微生物 |
Trichophyton indotineae IFM 67092, IFM 67093, IFM 67094, IFM 67095, IFM 67096, IFM 67097 |
