| RRC ID |
77859
|
| 著者 |
Ueki M, Suzuki T, Kato Y.
|
| タイトル |
Large-scale cultivation of human iPS cells in bioreactor with reciprocal mixing.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
A substantial number of human iPS cells (hiPSCs) is needed for cell therapy to be successful against various diseases. We previously reported on a bioreactor with reciprocal mixing that produces specific physical properties that differ from those of conventional bioreactors with rotary paddle stirring. Moreover, such reactors not only provide a homogeneous environment but also allow the control of spheroid size by changing the mixing speed. In this study, we applied this bioreactor to the large-scale cultivation of hiPSCs. Approximately 10 billion hiPSCs were obtained from 2.0 L of culture, and the high expression of pluripotency markers was maintained. Our findings indicate that a bioreactor with reciprocal mixing can be used for large-scale hiPSC cultivation.
|
| 公開日 |
2024-1-6
|
| DOI |
10.1016/j.jbiosc.2023.12.008
|
| PII |
S1389-1723(23)00372-9
|
| PMID |
38185598
|
| IF |
2.366
|
| リソース情報 |
| ヒト・動物細胞 |
201B7(HPS0063)
253G1(HPS0002)
HiPS-RIKEN-1A(HPS0003) |
