論文 - 詳細
| RRC ID | 77978 |
|---|---|
| 著者 | Iwamuro M, Shiraha H, Kobashi M, Horiguchi S, Okada H. |
| タイトル | Laminin 511-E8 Fragment Offers Superior Adhesion Properties for Gastric Cancer Cells Compared with Full-Length Laminin 511. |
| ジャーナル | Curr Issues Mol Biol |
| Abstract |
BACKGROUND:The interaction between cancer cells and laminin (Ln) is a key event in tumor invasion and metastasis. Previously, we determined the effect of full-length Ln511 on gastric cancer cells. However, the interactions between the Ln511-E8 fragment, a truncated protein of Ln511, and gastric cancer cells have not been investigated. METHODS:We investigated the adhesion properties of gastric cancer cells to full-length Ln511 and Ln511-E8 fragments. RESULTS:The proliferation of four gastric cancer cell lines (SH-10-TC, MKN74, SC-6-JCK, and MKN45) was highest on the Ln511-E8 fragment. Further, a larger cytoplasm was observed in SH-10-TC and MKN74 cells cultured on full-length Ln511 or Ln511-E8 fragments. The percentage of adhesive cells was highest on the Ln511-E8 fragment in all four cell lines. Moreover, adhesion of the gastric cancer cells to Ln511-E8 fragment-coated plates was reduced by the Cdc42 GTPase inhibitor in a dose-dependent manner, suggesting the involvement of Cdc42 in the Ln511-E8 fragment-induced enhanced adhesion of gastric cancer cells. CONCLUSIONS:The Ln511-E8 fragment had a greater impact on the adhesion, morphology, and proliferation of gastric cancer cells than full-length laminin. Thus, the Ln511-E8 fragment is suitable for investigating the interaction between gastric cancer cells and extracellular matrices in tumor invasion and metastasis. |
| 巻・号 | 44(4) |
| ページ | 1539-1551 |
| 公開日 | 2022-4-5 |
| DOI | 10.3390/cimb44040105 |
| PII | cimb44040105 |
| PMID | 35723363 |
| PMC | PMC9164087 |
| リソース情報 | |
| ヒト・動物細胞 | MKN74(RCB1002) MKN45(RCB1001) |