論文 - 詳細
| RRC ID | 78063 |
|---|---|
| 著者 | Carrillo-Carrasco VP, Hernández-García J, Weijers D. |
| タイトル | Electroporation-based delivery of proteins in Penium margaritaceum and other zygnematophycean algae. |
| ジャーナル | Physiol Plant |
| Abstract |
Zygnematophycean algae represent the streptophyte group identified as the closest sister clade to land plants. Their phylogenetic position and growing genomic resources make these freshwater algae attractive models for evolutionary studies in the context of plant terrestrialization. However, available genetic transformation protocols are limited and exclusively DNA-based. To expand the zygnematophycean toolkit, we developed a DNA-free method for protein delivery into intact cells using electroporation. We use confocal microscopy coupled with fluorescence lifetime imaging to assess the delivery of mNeonGreen into algal cells. We optimized the method to obtain high efficiency of delivery and cell recovery after electroporation in two strains of Penium margaritaceum and show that the experimental setup can also be used to deliver proteins in other zygnematophycean species such as Closterium peracerosum-strigosum-littorale complex and Mesotaenium endlicherianum. We discuss the possible applications of this proof-of-concept method. |
| 巻・号 | 175(6) |
| ページ | e14121 |
| 公開日 | 2023-1-1 |
| DOI | 10.1111/ppl.14121 |
| PMID | 38148204 |
| MeSH | Biological Evolution* Electroporation Phylogeny Plants* |
| IF | 4.148 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-67 NIES-217 |