| RRC ID |
78080
|
| 著者 |
Watabe T, Yamahira S, Takakura K, Thumkeo D, Narumiya S, Matsuda M, Terai K.
|
| タイトル |
Calcium transients trigger switch-like discharge of prostaglandin E2 in an extracellular signal-regulated kinase-dependent manner.
|
| ジャーナル |
Elife
|
| Abstract |
Prostaglandin E2 (PGE2) is a key player in a plethora of physiological and pathological events. Nevertheless, little is known about the dynamics of PGE2 secretion from a single cell and its effect on the neighboring cells. Here, by observing confluent Madin-Darby canine kidney (MDCK) epithelial cells expressing fluorescent biosensors, we demonstrate that calcium transients in a single cell cause PGE2-mediated radial spread of PKA activation (RSPA) in neighboring cells. By in vivo imaging, RSPA was also observed in the basal layer of the mouse epidermis. Experiments with an optogenetic tool revealed a switch-like PGE2 discharge in response to the increasing cytoplasmic Ca2+ concentrations. The cell density of MDCK cells correlated with the frequencies of calcium transients and the following RSPA. The extracellular signal-regulated kinase (ERK) activation also enhanced the frequency of RSPA in MDCK and in vivo. Thus, the PGE2 discharge is regulated temporally by calcium transients and ERK activity.
|
| 巻・号 |
12
|
| 公開日 |
2024-1-26
|
| DOI |
10.7554/eLife.86727
|
| PII |
86727
|
| PMID |
38276879
|
| PMC |
PMC10945702
|
| MeSH |
Animals
Calcium*
Dinoprostone
Dogs
Extracellular Signal-Regulated MAP Kinases*
Kidney
Mice
Phosphorylation
|
| リソース情報 |
| ヒト・動物細胞 |
MDCK(RCB0995) |
| 遺伝子材料 |
pCMV-VSV-G-RSV-Rev (RDB04393) |
