論文 - 詳細
| RRC ID | 78175 |
|---|---|
| 著者 | Itoh M, Kawagoe S, Nakagawa H, Asahina A, Okano HJ. |
| タイトル | Generation of induced pluripotent stem cell (iPSC) from NY-ESO-I-specific cytotoxic T cells isolated from the melanoma patient with minor HLAs: The practical pilot study for the adoptive immunotherapy for melanoma using iPSC technology. |
| ジャーナル | Exp Dermatol |
| Abstract |
Melanoma is one of the most severe skin cancers, derived from melanocytes. Among various therapies for melanoma, adoptive immunotherapy using tumor-infiltrating lymphocytes/chimeric antigen receptor-T cells (TCs) is advanced in recent years; however, the efficacy is still limited, and major challenges remain in terms of safety and cell supply. To solve the issues of adoptive immunotherapy, we utilized induced pluripotent stem cells (iPSCs), which have an unlimited proliferative ability and various differentiation capability. First, we monoclonally isolated CD8+ TCs specifically reactive with NY-ESO-1, one of tumor antigens, from the melanoma patient's monocytes after stimulated with NY-ESO-1 peptide by manual procedure, and cultured NY-ESO-1-specific TCs until proliferated and formed colonies. iPSCs were consequently generated from colony-forming TCs by exogenous expression of reprogramming factors using Sendai virus vector. After the RAG2 gene in TC-derived iPSCs (T-iPSCs) was knocked out for preventing T-cell receptor (TCR) rearrangement, T-iPSCs were re-differentiated into rejuvenated cytotoxic TCs. We confirmed that TCR of T-iPSC-derived TC was maintained as the same of original TCs. In conclusion, T-iPSCs have a potential to be an unlimited cell source for providing cytotoxic TCs. Our study could be a "touchstone" to develop iPSC-based adoptive immunotherapy for the treatment of melanoma for the future clinical use. |
| 巻・号 | 32(2) |
| ページ | 126-134 |
| 公開日 | 2023-2-1 |
| DOI | 10.1111/exd.14686 |
| PMID | 36222007 |
| MeSH | Antigens, Neoplasm CD8-Positive T-Lymphocytes / metabolism Humans Immunotherapy Immunotherapy, Adoptive Induced Pluripotent Stem Cells* / metabolism Melanoma* / pathology Pilot Projects Receptors, Antigen, T-Cell / metabolism T-Lymphocytes, Cytotoxic / metabolism |
| IF | 3.368 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | OP9/N-DLL1(RCB2927) |