論文 - 詳細
| RRC ID | 78177 |
|---|---|
| 著者 | Morinaga T, Iwatsuki M, Yamashita K, Yasuda-Yoshihara N, Yamane T, Matsumoto C, Harada K, Eto K, Kurashige J, Ishimoto T, Baba Y, Miyamoto Y, Yoshida N, Baba H. |
| タイトル | Dynamic Alteration in HLA-E Expression and Soluble HLA-E via Interaction with Natural Killer Cells in Gastric Cancer. |
| ジャーナル | Ann Surg Oncol |
| Abstract |
BACKGROUND:Some reports showed the immune tolerance of soluble human leukocyte antigen E (HLA-E), but the role that soluble HLA-E plays in gastric cancer (GC) is unknown. We aimed to clarify the molecular mechanism and clinical significance of soluble HLA-E in GC. METHODS:We examined the expression of HLA-E on GC cells and soluble HLA-E under co-culture with natural killer (NK) cells in a time-dependent manner. Changes in NK cell activity were investigated using anti-NK group 2 member A (NKG2A) antibodies in the presence of soluble HLA-E. Expression of soluble HLA-E in the serum of GC patients was determined. RESULTS:Whereas HLA-E expression on GC cells peaked with interferon (IFN)-γ secretion by NK cells in a time-dependent manner, soluble HLA-E was upregulated in conditioned medium. Pre-incubation with anti-NKG2A antibodies increased the activation of NKG2A+ NK cells in the presence of soluble HLA-E. Expression of soluble HLA-E in the serum of GC patients correlated with disease progression. CONCLUSIONS:HLA-E expression dynamically changes on GC cells and in conditioned medium. Furthermore, soluble HLA-E can contribute to immune escape in GC cell lines, which may have significance in clinical practice. Moreover, soluble HLA-E may be a potential prognostic biomarker. |
| 巻・号 | 30(2) |
| ページ | 1240-1252 |
| 公開日 | 2023-2-1 |
| DOI | 10.1245/s10434-022-12505-0 |
| PII | 10.1245/s10434-022-12505-0 |
| PMID | 36149610 |
| MeSH | Culture Media, Conditioned / metabolism HLA Antigens / metabolism HLA-E Antigens Histocompatibility Antigens Class I Humans Killer Cells, Natural Stomach Neoplasms* / metabolism |
| リソース情報 | |
| ヒト・動物細胞 | Kato III(RCB2088) |