RRC ID 78187
Author Ana Flávia Piquera Santos, Lara Cristina Cunha Cervantes, Roberta Okamoto, Antonio Carlos Guastaldi, Thallita Pereira Queiroz, Layla Panahipour, Reinhard Gruber, Francisley Ávila Souza
Title Interleukin-6 Expression of Osteogenic Cell Lines Grown on Laser-Treated and Hydroxyapatite-Coated Titanium Discs
Journal Applied Sciences
Abstract The laser treatment and hydroxyapatite coating of dental implants are supposed to enhance osseointegration, but prior to preclinical testing, any negative impact on cell viability should be ruled out. This study aimed to evaluate the response of murine osteogenic cell lineage MC3T3-E1 and the bone marrow-derived stromal cells ST2 to surface modifications of machined titanium discs, e.g., laser treatment without and with hydroxyapatite coating, as well as sandblasting followed by acid etching. Scanning electron microscopy and the contact angle measurements revealed that laser treatment caused a honeycomb surface and higher wettability compared to a machined or sandblasting acid-etched surface. Hydroxyapatite coating, however, not only reduced the viability of MC3T3-E1 and ST2 cells but also provoked the expression and release of interleukin-6. These findings suggest that the laser treatment of titanium supports its hydrophilicity, but adding hydroxyapatite can reduce cell viability and induce the concomitant release of inflammatory cytokines.
Volume 13
Pages 12646
Published 2023-11-24
DOI 10.3390/app132312646
Human and Animal Cells ST2(RCB0224)