論文 - 詳細
| RRC ID | 78189 |
|---|---|
| 著者 | Koyama Y, Fujihara S, Chiyo T, Matsui T, Hamaya S, Fujita K, Tani J, Morishita A, Kobara H, Ono M, Iwama H, Masaki T. |
| タイトル | Role of Mir-452-5p Overexpression in Epithelial-Mesenchymal Transition (EMT) in Early-stage Colorectal Cancer. |
| ジャーナル | In Vivo |
| Abstract |
BACKGROUND/AIM:The microRNA miR-452-5p holds a critical role in the progression of multiple tumor formations, but there is limited understanding regarding the epithelial-mesenchymal transition (EMT) progression and its underlying mechanisms in the early-stage colorectal cancer (CRC). We aimed to explore the change in miRNA expression in early-stage CRC and examine the role of these miRNAs in CRC. MATERIALS AND METHODS:The expression levels of miR-452-5p in tissues and cells of early-stage CRC were determined by real-time quantitative polymerase chain reaction. Additionally, the biological effects of miR-452-5p on CRC were investigated by in vitro functional experiments. RESULTS:The expression levels of miR-452-5p were found increased in early-stage CRC tissue. We found that miR-452-5p promoted CRC cell proliferation but inhibited epithelial-mesenchymal transition. Furthermore, miR-452-5p promoted cell proliferation through activation of the extracellular signal-regulated kinase pathway, and inhibited cell invasion through suppression of Slug (Snail2) expression and up-regulation of E-cadherin expression. CONCLUSION:The expression of miR-452-5p is up-regulated in early CRC and suppresses epithelial-mesenchymal transition in CRC. These discoveries suggest that miR-452-5p has the potential to serve as a viable therapeutic target for CRC. |
| 巻・号 | 37(5) |
| ページ | 1980-1990 |
| 公開日 | 2023-1-1 |
| DOI | 10.21873/invivo.13295 |
| PII | 37/5/1980 |
| PMID | 37652512 |
| PMC | PMC10500504 |
| MeSH | Cell Line, Tumor Cell Movement / genetics Cell Proliferation / genetics Colorectal Neoplasms* / pathology Epithelial-Mesenchymal Transition / genetics Gene Expression Regulation, Neoplastic Humans MicroRNAs* / genetics |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) COLO-320(RCB1193) CW-2(RCB0778) |