RRC ID 78189
Author Koyama Y, Fujihara S, Chiyo T, Matsui T, Hamaya S, Fujita K, Tani J, Morishita A, Kobara H, Ono M, Iwama H, Masaki T.
Title Role of Mir-452-5p Overexpression in Epithelial-Mesenchymal Transition (EMT) in Early-stage Colorectal Cancer.
Journal In Vivo
Abstract BACKGROUND/AIM:The microRNA miR-452-5p holds a critical role in the progression of multiple tumor formations, but there is limited understanding regarding the epithelial-mesenchymal transition (EMT) progression and its underlying mechanisms in the early-stage colorectal cancer (CRC). We aimed to explore the change in miRNA expression in early-stage CRC and examine the role of these miRNAs in CRC.
MATERIALS AND METHODS:The expression levels of miR-452-5p in tissues and cells of early-stage CRC were determined by real-time quantitative polymerase chain reaction. Additionally, the biological effects of miR-452-5p on CRC were investigated by in vitro functional experiments.
RESULTS:The expression levels of miR-452-5p were found increased in early-stage CRC tissue. We found that miR-452-5p promoted CRC cell proliferation but inhibited epithelial-mesenchymal transition. Furthermore, miR-452-5p promoted cell proliferation through activation of the extracellular signal-regulated kinase pathway, and inhibited cell invasion through suppression of Slug (Snail2) expression and up-regulation of E-cadherin expression.
CONCLUSION:The expression of miR-452-5p is up-regulated in early CRC and suppresses epithelial-mesenchymal transition in CRC. These discoveries suggest that miR-452-5p has the potential to serve as a viable therapeutic target for CRC.
Volume 37(5)
Pages 1980-1990
Published 2023-1-1
DOI 10.21873/invivo.13295
PII 37/5/1980
PMID 37652512
PMC PMC10500504
MeSH Cell Line, Tumor Cell Movement / genetics Cell Proliferation / genetics Colorectal Neoplasms* / pathology Epithelial-Mesenchymal Transition / genetics Gene Expression Regulation, Neoplastic Humans MicroRNAs* / genetics
Human and Animal Cells CACO-2(RCB0988) COLO-320(RCB1193) CW-2(RCB0778)