Reference - Detail
|Koyama Y, Fujihara S, Chiyo T, Matsui T, Hamaya S, Fujita K, Tani J, Morishita A, Kobara H, Ono M, Iwama H, Masaki T.
|Role of Mir-452-5p Overexpression in Epithelial-Mesenchymal Transition (EMT) in Early-stage Colorectal Cancer.
BACKGROUND/AIM:The microRNA miR-452-5p holds a critical role in the progression of multiple tumor formations, but there is limited understanding regarding the epithelial-mesenchymal transition (EMT) progression and its underlying mechanisms in the early-stage colorectal cancer (CRC). We aimed to explore the change in miRNA expression in early-stage CRC and examine the role of these miRNAs in CRC.
MATERIALS AND METHODS:The expression levels of miR-452-5p in tissues and cells of early-stage CRC were determined by real-time quantitative polymerase chain reaction. Additionally, the biological effects of miR-452-5p on CRC were investigated by in vitro functional experiments.
RESULTS:The expression levels of miR-452-5p were found increased in early-stage CRC tissue. We found that miR-452-5p promoted CRC cell proliferation but inhibited epithelial-mesenchymal transition. Furthermore, miR-452-5p promoted cell proliferation through activation of the extracellular signal-regulated kinase pathway, and inhibited cell invasion through suppression of Slug (Snail2) expression and up-regulation of E-cadherin expression.
CONCLUSION:The expression of miR-452-5p is up-regulated in early CRC and suppresses epithelial-mesenchymal transition in CRC. These discoveries suggest that miR-452-5p has the potential to serve as a viable therapeutic target for CRC.
|Cell Line, Tumor Cell Movement / genetics Cell Proliferation / genetics Colorectal Neoplasms* / pathology Epithelial-Mesenchymal Transition / genetics Gene Expression Regulation, Neoplastic Humans MicroRNAs* / genetics
|Human and Animal Cells
|CACO-2(RCB0988) COLO-320(RCB1193) CW-2(RCB0778)