| RRC ID |
78221
|
| Author |
Ito A, Matsuda N, Ukita Y, Okumura M, Chihara T.
|
| Title |
Akaluc/AkaLumine bioluminescence system enables highly sensitive, non-invasive and temporal monitoring of gene expression in Drosophila.
|
| Journal |
Commun Biol
|
| Abstract |
Bioluminescence generated by luciferase and luciferin has been extensively used in biological research. However, detecting signals from deep tissues in vivo poses a challenge to traditional methods. To overcome this, the Akaluc and AkaLumine bioluminescent systems were developed, resulting in improved signal detection. We evaluate the potential of Akaluc/AkaLumine in Drosophila melanogaster to establish a highly sensitive, non-invasive, and temporal detection method for gene expression. Our results show that oral administration of AkaLumine to flies expressing Akaluc provided a higher luminescence signal than Luc/D-luciferin, with no observed harmful effects on flies. The Akaluc/AkaLumine system allows for monitoring of dynamic temporal changes in gene expression. Additionally, using the Akaluc fusion gene allows for mRNA splicing monitoring. Our findings indicate that the Akaluc/AkaLumine system is a powerful bioluminescence tool for analyzing gene expression in deep tissues and small numbers of cells in Drosophila.
|
| Volume |
6(1)
|
| Pages |
1270
|
| Published |
2023-12-14
|
| DOI |
10.1038/s42003-023-05628-x
|
| PII |
10.1038/s42003-023-05628-x
|
| PMID |
38097812
|
| PMC |
PMC10721803
|
| MeSH |
Animals
Drosophila* / genetics
Drosophila melanogaster* / genetics
Drosophila melanogaster* / metabolism
Gene Expression
Luciferases / genetics
Luciferases / metabolism
Luciferins
Luminescent Measurements / methods
|
| IF |
4.165
|
| Resource |
| DNA material |
pcDNA3 Venus-Akaluc (RDB15781) |
| Drosophila |
|
