Reference - Detail
| RRC ID | 78221 |
|---|---|
| Author | Ito A, Matsuda N, Ukita Y, Okumura M, Chihara T. |
| Title | Akaluc/AkaLumine bioluminescence system enables highly sensitive, non-invasive and temporal monitoring of gene expression in Drosophila. |
| Journal | Commun Biol |
| Abstract |
Bioluminescence generated by luciferase and luciferin has been extensively used in biological research. However, detecting signals from deep tissues in vivo poses a challenge to traditional methods. To overcome this, the Akaluc and AkaLumine bioluminescent systems were developed, resulting in improved signal detection. We evaluate the potential of Akaluc/AkaLumine in Drosophila melanogaster to establish a highly sensitive, non-invasive, and temporal detection method for gene expression. Our results show that oral administration of AkaLumine to flies expressing Akaluc provided a higher luminescence signal than Luc/D-luciferin, with no observed harmful effects on flies. The Akaluc/AkaLumine system allows for monitoring of dynamic temporal changes in gene expression. Additionally, using the Akaluc fusion gene allows for mRNA splicing monitoring. Our findings indicate that the Akaluc/AkaLumine system is a powerful bioluminescence tool for analyzing gene expression in deep tissues and small numbers of cells in Drosophila. |
| Volume | 6(1) |
| Pages | 1270 |
| Published | 2023-12-14 |
| DOI | 10.1038/s42003-023-05628-x |
| PII | 10.1038/s42003-023-05628-x |
| PMID | 38097812 |
| PMC | PMC10721803 |
| MeSH | Animals Drosophila* / genetics Drosophila melanogaster* / genetics Drosophila melanogaster* / metabolism Gene Expression Luciferases / genetics Luciferases / metabolism Luciferins Luminescent Measurements / methods |
| IF | 4.165 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 12 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pcDNA3 Venus-Akaluc (RDB15781) |
| Drosophila | |