| RRC ID |
78238
|
| 著者 |
Ogiwara N, Nakano T, Masuda T, Kushiro K, Takai M.
|
| タイトル |
Cell Spheroid Formation on the Surface of Multi-Block Copolymers Composed of Poly(2-methoxyethyl acrylate) and Polyethylene Glycol.
|
| ジャーナル |
Macromol Biosci
|
| Abstract |
3D structured cells have great drug screening potential because they mimic in vivo tissues better than 2D cultured cells. In this study, multi-block copolymers composed of poly(2-methoxyethyl acrylate) (PMEA) and polyethylene glycol (PEG) are developed as a new kind of biocompatible polymers. PEG imparts non-cell adhesion while PMEA acts as an anchoring segment to prepare the polymer coating surface. The multi-block copolymers show higher stability in water than PMEA. A specific micro-sized swelling structure composed of a PEG chain is observed in the multi-block copolymer film in water. A single NIH3T3-3-4 spheroid is formed in 3 h on the surface of the multi-block copolymers with 8.4 wt% PEG. However, at a PEG content of 0.7 wt%, spheroid formed after 4 days. The adenosine triphosphate (ATP) activity of cells and the internal necrotic state of the spheroid change depending on PEG loading in the multi-block copolymers. As the formation rate of cell spheroid on low-PEG-ratio multi-block copolymers is slow, internal necrosis of cell spheroid is less likely to occur. Consequently, the cell spheroid formation rate by changing the PEG chain content in multi-block copolymers is successfully controlled. These unique surfaces are suggested to be useful for 3D cell culture.
|
| 巻・号 |
23(5)
|
| ページ |
e2200486
|
| 公開日 |
2023-5-1
|
| DOI |
10.1002/mabi.202200486
|
| PMID |
36880189
|
| MeSH |
Animals
Biocompatible Materials* / chemistry
Mice
NIH 3T3 Cells
Polyethylene Glycols* / chemistry
Polymers / chemistry
Water
|
| リソース情報 |
| ヒト・動物細胞 |
NIH3T3-3-4(RCB1862) |
