論文 - 詳細
| RRC ID | 78245 |
|---|---|
| 著者 | Ito F, Yanatori I, Kato K, Toyokuni S. |
| タイトル | Protocol for the isolation of GFP-expressing ferroptosis-dependent extracellular vesicles in in vitro cell culture models. |
| ジャーナル | STAR Protoc |
| Abstract |
Extracellular vesicles (EVs) are complex structures that transport various DNA, RNA, and protein. Recently, new EV secretion mechanisms have been identified through the iron regulatory system in mammalian cells. We revealed that ferroptosis increases EV secretion, which is named ferroptosis-dependent EVs (FedEVs). Here, we describe a step-by-step procedure to isolate GFP-expressing FedEVs for in vitro analysis. The FedEVs are further analyzed by imaging and flow cytometry analysis. For complete details on the use and execution of this protocol, please refer to Ito et al.1. |
| 巻・号 | 5(1) |
| ページ | 102892 |
| 公開日 | 2024-2-14 |
| DOI | 10.1016/j.xpro.2024.102892 |
| PII | S2666-1667(24)00057-1 |
| PMID | 38363686 |
| PMC | PMC10877193 |
| MeSH | Animals Cell Culture Techniques Extracellular Vesicles* / chemistry Ferroptosis* / genetics Mammals Proteins / metabolism |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) THP-1(RCB1189) |
| 遺伝子材料 | pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394) |