論文 - 詳細
| RRC ID | 78276 |
|---|---|
| 著者 | Vann K, Weidner AE, Walczyk AC, Astapova O. |
| タイトル | Paxillin knockout in mouse granulosa cells increases fecundity†. |
| ジャーナル | Biol Reprod |
| Abstract |
Paxillin is an intracellular adaptor protein involved in focal adhesions, cell response to stress, steroid signaling, and apoptosis in reproductive tissues. To investigate the role of paxillin in granulosa cells, we created a granulosa-specific paxillin knockout mouse model using Cre recombinase driven by the Anti-Müllerian hormone receptor 2 gene promoter. Female granulosa-specific paxillin knockout mice demonstrated increased fertility in later reproductive age, resulting in higher number of offspring when bred continuously up to 26 weeks of age. This was not due to increased numbers of estrous cycles, ovulated oocytes per cycle, or pups per litter, but this was due to shorter time to pregnancy and increased number of litters in the granulosa-specific paxillin knockout mice. The number of ovarian follicles was not significantly affected by the knockout at 30 weeks of age. Granulosa-specific paxillin knockout mice had slightly altered estrous cycles but no difference in circulating reproductive hormone levels. Knockout of paxillin using clustered regularly interspaced short palindromic repeat-associated protein 9 (CRISPR-Cas9) in human granulosa-derived immortalized KGN cells did not affect cell proliferation or migration. However, in cultured primary mouse granulosa cells, paxillin knockout reduced cell death under basal culture conditions. We conclude that paxillin knockout in granulosa cells increases female fecundity in older reproductive age mice, possibly by reducing granulosa cell death. This study implicates paxillin and its signaling network as potential granulosa cell targets in the management of age-related subfertility. |
| 巻・号 | 109(5) |
| ページ | 669-683 |
| 公開日 | 2023-11-15 |
| DOI | 10.1093/biolre/ioad093 |
| PII | 7238833 |
| PMID | 37552051 |
| PMC | PMC10651069 |
| MeSH | Aged Animals Female Fertility / genetics Granulosa Cells* / metabolism Humans Infant Mice Mice, Knockout Ovarian Follicle* / metabolism Paxillin / genetics Paxillin / metabolism Pregnancy |
| IF | 3.322 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | KGN(RCB1154) |