| RRC ID |
78291
|
| 著者 |
Sung TC, Chen YH, Wang T, Qian L, Chao WH, Liu J, Pang J, Ling QD, Lee HH, Higuchi A.
|
| タイトル |
Design of dual peptide-conjugated hydrogels for proliferation and differentiation of human pluripotent stem cells.
|
| ジャーナル |
Mater Today Bio
|
| Abstract |
Completely synthetic cell cultivation materials for human pluripotent stem cells (hPSCs) are important for the future clinical use of hPSC-derived cells. Currently, cell culture materials conjugated with extracellular matrix (ECM)-derived peptides are being prepared using only one specific integrin-targeting peptide. We designed dual peptide-conjugated hydrogels, for which each peptide was selected from different ECM sites: the laminin β4 chain and fibronectin or vitronectin, which can target α6β1 and α2β1 or αVβ5. hPSCs cultured on dual peptide-conjugated hydrogels, especially on hydrogels conjugated with peptides obtained from the laminin β4 chain and vitronectin with a low peptide concentration of 200 μg/mL, showed high proliferation ability over the long term and differentiated into cells originating from 3 germ layers in vivo as well as a specific lineage of cardiac cells. The design of grafting peptides was also important, for which a joint segment and positive amino acids were added into the designed peptide. Because of the designed peptides on the hydrogels, only 200 μg/mL peptide solution was sufficient for grafting on the hydrogels, and the hydrogels supported hPSC cultures long-term; in contrast, in previous studies, greater than 1000 μg/mL peptide solution was needed for the grafting of peptides on cell culture materials.
|
| 巻・号 |
25
|
| ページ |
100969
|
| 公開日 |
2024-4-1
|
| DOI |
10.1016/j.mtbio.2024.100969
|
| PII |
S2590-0064(24)00028-0
|
| PMID |
38318478
|
| PMC |
PMC10839443
|
| リソース情報 |
| ヒト・動物細胞 |
454E2(HPS0077) |
