| RRC ID |
78320
|
| 著者 |
Ishinaga H, Okugawa Y, Hou B, He F, Yin C, Murata M, Toiyama Y, Takeuchi K.
|
| タイトル |
The role of miR-21 as a predictive biomarker and a potential target to improve the effects of chemoradiotherapy against head and neck squamous cell carcinoma.
|
| ジャーナル |
J Radiat Res
|
| Abstract |
This study aimed to clarify whether circulating miR-21 represents a predictive biomarker in patients with head and neck squamous cell carcinoma (HNSCC) undergoing chemoradiotherapy, and to investigate the effect of miR-21 inhibitor for chemoradiation in human SCC cells. Plasma samples were obtained from 22 patients with HNSCC and 25 non-cancer volunteers. Plasma miR-21 expression was measured using real-time quantitative reverse transcription polymerase chain reaction. The effects of miR-21 inhibitor in human SCC cells were investigated by performing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry and western blot analysis. As a result, plasma miR-21 expression was higher in HNSCC patients than in control patients (P < 0.001). Seven patients with recurrence showed significantly higher plasma miR-21 than the 15 patients without recurrence. And high miR-21 expression group showed poor overall survival. Moreover, miR-21 inhibition significantly enhanced cisplatin- or radiation-induced apoptosis. Western blot analysis suggested the programmed cell death 4 protein as a potential target of miR-21 in relation to apoptosis. In conclusion, this study provides new insights into the role of miR-21 as a predictive biomarker for HNSCC treated with chemoradiotherapy and suggests a potential target to improve the effects of chemoradiotherapy against HNSCC.
|
| 巻・号 |
64(4)
|
| ページ |
668-676
|
| 公開日 |
2023-7-18
|
| DOI |
10.1093/jrr/rrad043
|
| PII |
7197081
|
| PMID |
37312615
|
| PMC |
PMC10354857
|
| MeSH |
Biomarkers, Tumor
Carcinoma, Squamous Cell*
Cell Line, Tumor
Chemoradiotherapy
Gene Expression Regulation, Neoplastic
Head and Neck Neoplasms* / therapy
Humans
MicroRNAs* / genetics
MicroRNAs* / metabolism
Squamous Cell Carcinoma of Head and Neck / therapy
|
| リソース情報 |
| ヒト・動物細胞 |
SAS(RCB1974) |
