Reference - Detail
| RRC ID | 78335 |
|---|---|
| Author | Jo-Watanabe A, Inaba T, Osada T, Hashimoto R, Nishizawa T, Okuno T, Ihara S, Touhara K, Hattori N, Oh-Hora M, Nureki O, Yokomizo T. |
| Title | Bicarbonate signalling via G protein-coupled receptor regulates ischaemia-reperfusion injury. |
| Journal | Nat Commun |
| Abstract |
Homoeostatic regulation of the acid-base balance is essential for cellular functional integrity. However, little is known about the molecular mechanism through which the acid-base balance regulates cellular responses. Here, we report that bicarbonate ions activate a G protein-coupled receptor (GPCR), i.e., GPR30, which leads to Gq-coupled calcium responses. Gpr30-Venus knock-in mice reveal predominant expression of GPR30 in brain mural cells. Primary culture and fresh isolation of brain mural cells demonstrate bicarbonate-induced, GPR30-dependent calcium responses. GPR30-deficient male mice are protected against ischemia-reperfusion injury by a rapid blood flow recovery. Collectively, we identify a bicarbonate-sensing GPCR in brain mural cells that regulates blood flow and ischemia-reperfusion injury. Our results provide a perspective on the modulation of GPR30 signalling in the development of innovative therapies for ischaemic stroke. Moreover, our findings provide perspectives on acid/base sensing GPCRs, concomitantly modulating cellular responses depending on fluctuating ion concentrations under the acid-base homoeostasis. |
| Volume | 15(1) |
| Pages | 1530 |
| Published | 2024-2-27 |
| DOI | 10.1038/s41467-024-45579-3 |
| PII | 10.1038/s41467-024-45579-3 |
| PMID | 38413581 |
| PMC | PMC10899177 |
| MeSH | Animals Bicarbonates Brain Ischemia* Calcium / metabolism Male Mice Receptors, Estrogen / metabolism Receptors, G-Protein-Coupled / genetics Receptors, G-Protein-Coupled / metabolism Reperfusion Injury* Stroke* |
| IF | 12.121 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 49 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | C2C12(RCB0987) |