論文 - 詳細
| RRC ID | 78372 |
|---|---|
| 著者 | Shi L, Ura K, Takagi Y. |
| タイトル | Effects of self-assembled type II collagen fibrils on the morphology and growth of pre-chondrogenic ATDC5 cells. |
| ジャーナル | Osteoarthr Cartil Open |
| Abstract |
OBJECTIVE:Although type II collagen could have marked potential for developing cartilage tissue engineering (CTE) scaffolds, its erratic supply and viscous nature have limited these studies, and there are no studies on the use of marine-derived type II collagen fibrils for CTE scaffold materials. In this study, we aimed to generate a fibril-based, thin-layered scaffold from marine-derived type II collagen and investigate its chondrogenic potential. METHODS:Time-lapse observations revealed the cell adhesion process. The Cell Counting Kit-8 (CCK-8) assay, light microscopy, and scanning electron microscopy were performed to detect proliferation and filopodium morphology. Alcian blue staining was used to show the deposition of extracellular secretions, and qRT-PCR was performed to reveal the expression levels of chondrogenesis-related genes. RESULTS:The cell adhesion speed was similar in both fibril-coated and control molecule-coated groups, but the cellular morphology, proliferation, and chondrogenesis activity differed. On fibrils, more elongated finer filopodia showed inter-cell communications, whereas the slower proliferation suggested an altered cell cycle. Extracellular secretions occurred before day 14 and continued until day 28 on fibrils, and on fibrils, the expression of the chondrogenesis-related genes Sox9 (p < 0.001), Col10a1 (p < 0.001), Acan (p < 0.001), and Col2a1 (p = 0.0049) was significantly upregulated on day 21. CONCLUSION:Marine-derived type II collagen was, for the first time, fabricated into a fibril state. It showed rapid cellular affinity and induced chondrogenesis with extracellular secretions. We presented a new model for studying chondrogenesis in vitro and a potential alternative material for cell-laden CTE research. |
| 巻・号 | 6(2) |
| ページ | 100450 |
| 公開日 | 2024-6-1 |
| DOI | 10.1016/j.ocarto.2024.100450 |
| PII | S2665-9131(24)00017-7 |
| PMID | 38444516 |
| PMC | PMC10914481 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |