| RRC ID |
78421
|
| Author |
Crawford M, Barr K, Dagnino L.
|
| Title |
Analysis of Proliferation, Apoptosis, and Motility in Mouse Embryonic Melanocytic Precursor Cells.
|
| Journal |
Methods Mol Biol
|
| Abstract |
In this chapter, we provide a method to purify and culture embryonic melanocytic stem cells that express green fluorescent protein in a cell-type specific manner. Isolation of melanocytic lineage cell populations that are >98% pure is accomplished through the use of GFP-based fluorescence activated cell sorting. We also provide a method to culture the purified melanoblasts and to analyze their proliferation, apoptosis, and motility properties.
|
| Published |
2024-3-6
|
| DOI |
10.1007/7651_2024_528
|
| PMID |
38441720
|
| Resource |
| Human and Animal Cells |
ST2(RCB0224) |
