| RRC ID |
78423
|
| 著者 |
Munekane M, Mori H, Takada N, Sano K, Yamasaki T, Tanaka T, Sasaki N, Rikitake Y, Mukai T.
|
| タイトル |
Preparation and evaluation of 111In-labeled liposomes containing phosphatidylglycerol for detection of macrophages in atherosclerotic plaques.
|
| ジャーナル |
Nucl Med Biol
|
| Abstract |
Macrophage infiltration is a characteristic feature of atherosclerotic plaque progression. Since liposomes containing 1,2-distearoyl-sn-glycero-3-phosphoglycerol (DSPG) are efficiently phagocytosed by macrophages, we deduced that radiolabeled liposomes containing DSPG could potentially be used for nuclear imaging of vulnerable atherosclerotic plaques. Indium-111 (111In)-labeled liposomes containing different ratios of DSPG were developed with a high labeling efficiency. 111In-labeled liposomes with higher DSPG content showed higher uptake by macrophage-like RAW264 cells. A biodistribution study demonstrated rapid blood clearance and selective accumulation in the liver and spleen, especially in normal mice injected with 111In-labeled liposomes with higher DSPG content. Accumulation in atherosclerotic plaques was evaluated using 111In-labeled DSPG liposomes, which had the highest DSPG content among the studied liposomes. 111In-labeled DSPG liposomes accumulated in the plaques and the radioactive regions were mostly consistent with the distribution of macrophages. The target-to-non-target ratio of 111In-labeled DSPG liposomes was higher than that of 111In-labeled control liposomes without DSPG. These results suggest that 111In-labeled liposomes containing DSPG are useful for nuclear medical diagnosis of atherosclerotic plaques.
|
| 巻・号 |
126-127
|
| ページ |
108388
|
| 公開日 |
2023-1-1
|
| DOI |
10.1016/j.nucmedbio.2023.108388
|
| PII |
S0969-8051(23)00076-8
|
| PMID |
37804560
|
| MeSH |
Animals
Liposomes
Macrophages
Mice
Phosphatidylglycerols
Plaque, Atherosclerotic* / diagnostic imaging
Tissue Distribution
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
