RRC ID 78442
Author Fujibayashi S, Kiyono T, Endo Y, Tani T, Tate H, Bai L, Sugano E, Tomita H, Fukuda T.
Title Increased lentivirus titer using ultra expression vectors.
Journal Anal Biochem
Abstract Lentivirus is an efficient gene transfer system that is widely used in basic science. We aimed to improve viral titer by applying an ultra-expression vectors to lentiviral packaging. Application of the ultra-expression vectors increased biological titer 4 times for standard preparation. We also evaluated the efficacy of the ultra-expression vectors to relatively longer insert fragments, such as CSII-CMV-CNROE containing 5 genes in multiple cloning sites. Packaging of the ultra-expression vectors showed 3.5 times higher biological titer compared with the original method. Our improved packaging system could be applied to lentivirus to produce higher titers.
Volume 669
Pages 115119
Published 2023-5-15
DOI 10.1016/j.ab.2023.115119
PII S0003-2697(23)00084-2
PMID 36958509
MeSH Base Sequence Genetic Vectors* / genetics Lentivirus* / genetics Lentivirus* / metabolism Transduction, Genetic
Human and Animal Cells HE16(RCB2237)