Reference - Detail
| RRC ID | 78462 |
|---|---|
| Author | Shou J, Komazawa A, Wachi Y, Kawatani M, Fujioka H, Spratt SJ, Mizuguchi T, Oguchi K, Akaboshi H, Obata F, Tachibana R, Yasunaga S, Mita Y, Misawa Y, Kojima R, Urano Y, Kamiya M, Ozeki Y. |
| Title | Super-resolution vibrational imaging based on photoswitchable Raman probe. |
| Journal | Sci Adv |
| Abstract |
Super-resolution vibrational microscopy is promising to increase the degree of multiplexing of nanometer-scale biological imaging because of the narrower spectral linewidth of molecular vibration compared to fluorescence. However, current techniques of super-resolution vibrational microscopy suffer from various limitations including the need for cell fixation, high power loading, or complicated detection schemes. Here, we present reversible saturable optical Raman transitions (RESORT) microscopy, which overcomes these limitations by using photoswitchable stimulated Raman scattering (SRS). We first describe a bright photoswitchable Raman probe (DAE620) and validate its signal activation and depletion characteristics when exposed to low-power (microwatt level) continuous-wave laser light. By harnessing the SRS signal depletion of DAE620 through a donut-shaped beam, we demonstrate super-resolution vibrational imaging of mammalian cells with excellent chemical specificity and spatial resolution beyond the optical diffraction limit. Our results indicate RESORT microscopy to be an effective tool with high potential for multiplexed super-resolution imaging of live cells. |
| Volume | 9(24) |
| Pages | eade9118 |
| Published | 2023-6-16 |
| DOI | 10.1126/sciadv.ade9118 |
| PMID | 37327330 |
| PMC | PMC10275589 |
| MeSH | Animals Mammals Microscopy* / methods Spectrum Analysis, Raman / methods Vibration* |
| IF | 13.117 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 38 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) |