RRC ID 78479
Author Yoshida T, Yokoi T, Tanaka T, Matsuzaka E, Saida Y, Nishina S, Takada S, Shimizu S, Azuma N.
Title Modeling of Retina and Optic Nerve Ischemia-Reperfusion Injury through Hypoxia-Reoxygenation in Human Induced Pluripotent Stem Cell-Derived Retinal Ganglion Cells.
Journal Cells
Abstract Retinal ganglion cells (RGCs) are specialized projection neurons that constitute part of the retina, and the death of RGCs causes various eye diseases, but the mechanism of RGC death is still unclear. Here, we induced cell death in human induced pluripotent stem cell (hiPSC)-derived RGC-rich retinal tissues using hypoxia-reoxygenation in vitro. Flow cytometry, immunochemistry, and Western blotting showed the apoptosis and necrosis of RGCs under hypoxia-reoxygenation, and they were rescued by an apoptosis inhibitor but not by a necrosis inhibitor. This revealed that the cell death induced in our model was mainly due to apoptosis. To our knowledge, this is the first model to reproduce ischemia-reperfusion in hiPSC-derived RGCs. Thus, the efficacy of apoptosis inhibitors and neuroprotective agents can be evaluated using this model, bringing us closer to clinical applications.
Volume 13(2)
Published 2024-1-11
DOI 10.3390/cells13020130
PII cells13020130
PMID 38247823
PMC PMC10814087
MeSH Humans Hypoxia Induced Pluripotent Stem Cells* Necrosis Optic Nerve Optic Neuropathy, Ischemic* Reperfusion Injury* Retina Retinal Ganglion Cells
Resource
Human and Animal Cells human iPSCs