RRC ID 78489
Author Tojima T, Suda Y, Jin N, Kurokawa K, Nakano A.
Title Spatiotemporal dissection of the Golgi apparatus and the ER-Golgi intermediate compartment in budding yeast.
Journal Elife
Abstract Cargo traffic through the Golgi apparatus is mediated by cisternal maturation, but it remains largely unclear how the cis-cisternae, the earliest Golgi sub-compartment, is generated and how the Golgi matures into the trans-Golgi network (TGN). Here, we use high-speed and high-resolution confocal microscopy to analyze the spatiotemporal dynamics of a diverse set of proteins that reside in and around the Golgi in budding yeast. We find many mobile punctate structures that harbor yeast counterparts of mammalian endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) proteins, which we term 'yeast ERGIC'. It occasionally exhibits approach and contact behavior toward the ER exit sites and gradually matures into the cis-Golgi. Upon treatment with the Golgi-disrupting agent brefeldin A, the ERGIC proteins form larger aggregates corresponding to the Golgi entry core compartment in plants, while cis- and medial-Golgi proteins are absorbed into the ER. We further analyze the dynamics of several late Golgi proteins to better understand the Golgi-TGN transition. Together with our previous studies, we demonstrate a detailed spatiotemporal profile of the entire cisternal maturation process from the ERGIC to the Golgi and further to the TGN.
Volume 13
Published 2024-3-19
DOI 10.7554/eLife.92900
PII 92900
PMID 38501165
PMC PMC10950332
MeSH Animals Endoplasmic Reticulum / metabolism Golgi Apparatus / metabolism Mammals Saccharomyces cerevisiae* / metabolism Saccharomycetales* trans-Golgi Network / metabolism
Resource
Human and Animal Cells HeLa(RCB0007)