論文 - 詳細
| RRC ID | 78511 |
|---|---|
| 著者 | Izumi Y, Ohta N, Hisata K, Raabe T, Matsuzaki F. |
| タイトル | Drosophila Pins-binding protein Mud regulates spindle-polarity coupling and centrosome organization. |
| ジャーナル | Nat Cell Biol |
| Abstract |
The orientation of the mitotic spindle relative to the cell axis determines whether polarized cells undergo symmetric or asymmetric divisions. Drosophila epithelial cells and neuroblasts provide an ideal pair of cells to study the regulatory mechanisms involved. Epithelial cells divide symmetrically, perpendicular to the apical-basal axis. In the asymmetric divisions of neuroblasts, by contrast, the spindle reorients parallel to that axis, leading to the unequal distribution of cell-fate determinants to one daughter cell. Receptor-independent G-protein signalling involving the GoLoco protein Pins is essential for spindle orientation in both cell types. Here, we identify Mushroom body defect (Mud) as a downstream effector in this pathway. Mud directly associates and colocalizes with Pins at the cell cortex overlying the spindle pole(s) in both neuroblasts and epithelial cells. The cortical Mud protein is essential for proper spindle orientation in the two different division modes. Moreover, Mud localizes to centrosomes during mitosis independently of Pins to regulate centrosomal organization. We propose that Drosophila Mud, vertebrate NuMA and Caenorhabditis elegans Lin-5 (refs 5, 6) have conserved roles in the mechanism by which G-proteins regulate the mitotic spindle. |
| 巻・号 | 8(6) |
| ページ | 586-93 |
| 公開日 | 2006-6-1 |
| DOI | 10.1038/ncb1409 |
| PII | ncb1409 |
| PMID | 16648846 |
| MeSH | Animals Cell Cycle Proteins Cell Polarity Centrosome* Drosophila Drosophila Proteins / metabolism* Drosophila Proteins / physiology* Epithelial Cells / cytology GTP-Binding Proteins / physiology Guanine Nucleotide Dissociation Inhibitors / metabolism* Membrane Proteins / metabolism Membrane Proteins / physiology* Nerve Tissue Proteins / metabolism Nerve Tissue Proteins / physiology* Neurons / cytology Protein Binding Spindle Apparatus* |
| IF | 20.042 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#119097 DGRC#119098 |