論文 - 詳細
| RRC ID | 78622 |
|---|---|
| 著者 | Takimoto Y, Tsubamoto H, Isono-Taniguchi R, Ueda T, Sakata K, Nakagawa K, Narita S, Wakimoto YU, Shibahara H, Nishiumi S. |
| タイトル | Itraconazole Modulates Phospholipid Levels in Tumor-associated Macrophages. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Itraconazole, an antifungal drug, repolarizes pro-tumorigenic M2 tumor-associated macrophages to anti-tumorigenic M1-like phenotypes, thereby inhibiting the proliferation of cancer cells; however, the underlying mechanism remains unclear. Therefore, we investigated the effect of itraconazole on membrane-associated lipids in tumor-associated macrophages (TAM). MATERIALS AND METHODS:M1 and M2 macrophages were derived from the human monocyte leukemia cell line (THP-1) and cultured with or without 10 μM itraconazole. Cells were homogenized and subjected to liquid chromatography/mass spectrometry (LC/MS) analysis to estimate the glycerophospholipid levels in the cells. RESULTS:Lipidomic analysis results, displayed on a volcano plot, revealed that itraconazole-induced altered phospholipid composition, with more pronounced changes in M2 macrophages than in M1. Notably, itraconazole significantly increased intracellular phosphatidylinositol and lysophosphatidylcholine levels in M2 macrophages. CONCLUSION:Itraconazole modulates the lipid metabolism of TAMs, which could have implications for the development of novel cancer therapies. |
| 巻・号 | 43(5) |
| ページ | 1981-1984 |
| 公開日 | 2023-5-1 |
| DOI | 10.21873/anticanres.16358 |
| PII | 43/5/1981 |
| PMID | 37097657 |
| MeSH | Cell Differentiation Cell Line, Tumor Humans Itraconazole* / pharmacology Macrophages / metabolism Phospholipids / metabolism Phospholipids / pharmacology Tumor Microenvironment Tumor-Associated Macrophages* |
| リソース情報 | |
| ヒト・動物細胞 | THP-1 |