論文 - 詳細
| RRC ID | 78628 |
|---|---|
| 著者 | Hirata R, Iwata T, Fujita T, Nagahara T, Matsuda S, Sasaki S, Taniguchi Y, Hamamoto Y, Ouhara K, Kudo Y, Kurihara H, Mizuno N. |
| タイトル | Periostin regulates integrin expression in gingival epithelial cells. |
| ジャーナル | J Oral Biosci |
| Abstract |
OBJECTIVE:Human gingival epithelial cells (HGECs) function as a mechanical barrier against invasion by pathogenic organisms through epithelial cell-cell junction complexes, which are complex components of integrin. Integrins play an important role in the protective functions of HGECs. Human periodontal ligament (HPL) cells regulate periodontal homeostasis. However, periodontitis results in the loss of HPL cells. Therefore, as replenishment, HPL cells or mesenchymal stem cells (MSCs) can be transplanted. Herein, HPL cells and MSCs were used to elucidate the regulatory mechanisms of HGECs, assuming periodontal tissue homeostasis. METHODS:Human gingival fibroblasts (HGFs), HGECs, HPL cells, and MSCs were cultured, and the conditioned medium was collected. With or without silencing periostin mRNA, HGECs were cultured under normal conditions or in a conditioned medium. Integrin and periostin mRNA expression was determined using real-time polymerase chain reaction. Integrin protein expression was analyzed using flow cytometry, and periostin protein expression was determined via western blotting. RESULTS:The conditioned medium affected integrin expression in HGECs. Higher expression of periostin was observed in MSCs and HPL cells than in HGFs. The conditioned medium that contained periostin protein regulated integrin expression in HGECs. After silencing periostin in MSCs and HPL cells, periostin protein was not detected in the conditioned medium, and integrin expression in HGECs remained unaffected. CONCLUSIONS:Integrins in HGECs are regulated by periostin secreted from HPL cells and MSCs. This result suggests that periostin maintains gingival cell adhesion and regulates bacterial invasion/infection. Therefore, the functional regulation of periostin-secreting cells is important in preventing periodontitis. |
| 巻・号 | 66(1) |
| ページ | 170-178 |
| 公開日 | 2024-3-1 |
| DOI | 10.1016/j.job.2023.11.009 |
| PII | S1349-0079(23)00190-1 |
| PMID | 38048847 |
| MeSH | Culture Media, Conditioned / metabolism Culture Media, Conditioned / pharmacology Epithelial Cells / metabolism Humans Integrins / genetics Integrins / metabolism Periodontitis* Periostin* RNA, Messenger / genetics RNA, Messenger / metabolism |
| リソース情報 | |
| ヒト・動物細胞 | human mesenchymal stem cells |