論文 - 詳細
| RRC ID | 78709 |
|---|---|
| 著者 | Romer J, Gutbrod K, Schuppener A, Melzer M, Müller-Schüssele SJ, Meyer AJ, Dörmann P. |
| タイトル | Tocopherol and phylloquinone biosynthesis in chloroplasts requires the phytol kinase VITAMIN E PATHWAY GENE5 (VTE5) and the farnesol kinase (FOLK). |
| ジャーナル | Plant Cell |
| Abstract |
Chlorophyll degradation causes the release of phytol, which is converted into phytyl diphosphate (phytyl-PP) by phytol kinase (VITAMIN E PATHWAY GENE5 [VTE5]) and phytyl phosphate (phytyl-P) kinase (VTE6). The kinase pathway is important for tocopherol synthesis, as the Arabidopsis (Arabidopsis thaliana) vte5 mutant contains reduced levels of tocopherol. Arabidopsis harbors one paralog of VTE5, farnesol kinase (FOLK) involved in farnesol phosphorylation. Here, we demonstrate that VTE5 and FOLK harbor kinase activities for phytol, geranylgeraniol, and farnesol with different specificities. While the tocopherol content of the folk mutant is unchanged, vte5-2 folk plants completely lack tocopherol. Tocopherol deficiency in vte5-2 plants can be complemented by overexpression of FOLK, indicating that FOLK is an authentic gene of tocopherol synthesis. The vte5-2 folk plants contain only ∼40% of wild-type amounts of phylloquinone, demonstrating that VTE5 and FOLK both contribute in part to phylloquinone synthesis. Tocotrienol and menaquinone-4 were produced in vte5-2 folk plants after supplementation with homogentisate or 1,4-dihydroxy-2-naphthoic acid, respectively, indicating that their synthesis is independent of the VTE5/FOLK pathway. These results show that phytyl moieties for tocopherol synthesis are completely but, for phylloquinone production, only partially derived from geranylgeranyl-chlorophyll and phytol phosphorylation by VTE5 and FOLK. |
| 巻・号 | 36(4) |
| ページ | 1140-1158 |
| 公開日 | 2024-3-29 |
| DOI | 10.1093/plcell/koad316 |
| PII | 7485692 |
| PMID | 38124486 |
| PMC | PMC10980339 |
| MeSH | Arabidopsis* / genetics Arabidopsis* / metabolism Chlorophyll / metabolism Chloroplasts / genetics Chloroplasts / metabolism Farnesol / metabolism Phosphotransferases (Alcohol Group Acceptor)* Phytol / metabolism Plants / metabolism Tocopherols* / metabolism Vitamin E / metabolism Vitamin K 1 / metabolism |
| IF | 9.618 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pst12490 |