RRC ID 78772
Author Bai Y, Akiyama Y, Nagasaki H, Yagi OK, Kikuchi Y, Saito N, Takeshita K, Iwai T, Yuasa Y.
Title Distinct expression of CDX2 and GATA4/5, development-related genes, in human gastric cancer cell lines.
Journal Mol Carcinog
Abstract CDX2 is a tumor-suppressor homeobox gene involved in colon carcinogenesis, but its role in gastric cancer is unknown. Although GATA4, -5 and, -6 transcription factors have distinct functions in the regulation of gastrointestinal epithelial cell differentiation, there have been no reports regarding GATA4/5/6 alterations in gastrointestinal carcinomas. By using a semiquantitative reverse transcription-polymerase chain reaction assay, we studied the expression of gut development-related genes CDX2/1 and GATA4/5/6 in 11 human gastric cancer cell lines. The expression of CDX2 appeared to progressively decrease with the transition from well differentiated to poorly differentiated cancer cell lines. CDX1 was below detectable levels in all cell lines. The expression of GATA4 and GATA5 was undetectable in four and six cell lines, respectively, whereas the majority of the cell lines expressed GATA6 abundantly. These results suggest that CDX2 and GATA4/5 may be associated with the carcinogenesis of the stomach. Mol. Carcinog. 28:184-188, 2000.
Volume 28(3)
Pages 184-8
Published 2000-7-1
DOI 10.1002/1098-2744(200007)28:3<184::aid-mc7>3.0.co;2-6
PII 10.1002/1098-2744(200007)28:3<184::AID-MC7>3.0.CO;2-6
PMID 10942535
MeSH Adenocarcinoma / genetics Adenocarcinoma / metabolism Adenocarcinoma / pathology* Avian Proteins* CDX2 Transcription Factor Cell Differentiation / genetics Cell Transformation, Neoplastic / genetics DNA-Binding Proteins / biosynthesis* DNA-Binding Proteins / genetics GATA4 Transcription Factor GATA5 Transcription Factor Gene Expression Regulation, Neoplastic* Homeodomain Proteins / biosynthesis* Homeodomain Proteins / genetics Humans Immunoenzyme Techniques Neoplasm Proteins / biosynthesis* Neoplasm Proteins / genetics Reverse Transcriptase Polymerase Chain Reaction Stomach Neoplasms / genetics Stomach Neoplasms / metabolism Stomach Neoplasms / pathology* Transcription Factors / biosynthesis* Transcription Factors / genetics
IF 4.123
Human and Animal Cells MKN7(RCB0999) MKN28(RCB1000) MKN45(RCB1001) MKN74(RCB1002) GCIY(RCB0555)