論文 - 詳細
| RRC ID | 78811 |
|---|---|
| 著者 | Murokawa H, Egusa K, Shibutani S, Iwata H. |
| タイトル | Mechanistic/mammalian target of rapamycin complex 1 (mTORC1) signaling is involved in phagocytosis activation during THP-1 cell differentiation. |
| ジャーナル | J Vet Med Sci |
| Abstract |
Mechanistic/mammalian target of rapamycin complex 1 (mTORC1) is a serine/threonine kinase that plays a major role in cell metabolism. Although mTORC1 inhibitors are known to exert immunosuppressive effects, their effects on immune cells are not fully understood. In the present study, we examined the involvement of mTORC1 in the differentiation and functions of macrophages using THP-1 cells, which are derived from human monocytic leukemia and differentiate into macrophage-like cells upon treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA). We also examined the effects of two mTOR inhibitors, Torin 1 and rapamycin, on TPA-stimulated THP-1 cells. Although mTORC1 activation was observed upon TPA stimulation, mTOR inhibitors did not affect TPA-induced morphological changes or expression of the general macrophage marker, CD11b. In contrast, phagocytosis and fluid endocytosis were significantly impaired by the mTOR inhibitors. Endocytosis suppression was observed when mTOR inhibitors were added during differentiation, but not before or after differentiation, suggesting that endocytosis suppression altered the direction of differentiation. Furthermore, mTOR inhibitors altered the expression of M1/M2 polarization markers. These results suggest that the immunosuppressive effects of mTOR inhibitors may involve the suppression of macrophage endocytosis caused by abnormal cell differentiation. |
| 巻・号 | 85(7) |
| ページ | 772-780 |
| 公開日 | 2023-7-17 |
| DOI | 10.1292/jvms.22-0504 |
| PMID | 37225457 |
| PMC | PMC10372260 |
| MeSH | Animals Cell Differentiation Humans MTOR Inhibitors* Mammals Mechanistic Target of Rapamycin Complex 1 / metabolism Phagocytosis THP-1 Cells TOR Serine-Threonine Kinases* / metabolism |
| IF | 1.049 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | THP-1(RCB1189) |